• Product nameAnti-BTK antibody
    See all BTK primary antibodies
  • Description
    Rabbit polyclonal to BTK
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to 16 N terminal amino acids near the amino terminus of Human BTK.

  • Positive control
    • U937 cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide.
    Constituents: PBS.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesBTK Antibody is affinity chromatography purified via peptide column.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab25971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 73 kDa.Can be blocked with Human BTK peptide (ab39736).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.


  • FunctionPlays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
  • Involvement in diseaseDefects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
    Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
  • Cellular localizationCytoplasm. Membrane. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Agammaglobulinaemia tyrosine kinase antibody
    • AGMX 1 antibody
    • AGMX1 antibody
    • AT antibody
    • ATK antibody
    • B cell progenitor kinase antibody
    • B-cell progenitor kinase antibody
    • BPK antibody
    • Bruton agammaglobulinemia tyrosine kinase antibody
    • Bruton tyrosine kinase antibody
    • Bruton’s Tyrosine Kinase antibody
    • Btk antibody
    • BTK_HUMAN antibody
    • dominant-negative kinase-deficient Brutons tyrosine kinase antibody
    • IMD 1 antibody
    • IMD1 antibody
    • MGC126261 antibody
    • MGC126262 antibody
    • OTTHUMP00000063593 antibody
    • PSCTK 1 antibody
    • PSCTK1 antibody
    • truncated Bruton agammaglobulinemia tyrosine kinase antibody
    • Tyrosine protein kinase BTK antibody
    • Tyrosine-protein kinase BTK antibody
    • tyrosine-protein kinase BTK isoform (lacking exon 14 antibody
    • XLA antibody
    see all

Anti-BTK antibody images

  • Immunohistochemistry of BTK in mouse brain tissue with BTK antibody at 5 µg/ml.

  • Lane 1 : Anti-BTK antibody (ab25971) at 0.5 µg/ml
    Lane 2 : Anti-BTK antibody (ab25971) at 1 µg/ml
    Lane 3 : Anti-BTK antibody (ab25971) at 2 µg/ml

    Lane 1 : for analysis of U937 cell lysate (15ug lysate/lane)
    Lane 2 : for analysis of U937 cell lysate (15ug lysate/lane)
    Lane 3 : for analysis of U937 cell lysate (15ug lysate/lane)

    Predicted band size : 73 kDa
  • ab52971 at 10µg/ml staining BTK in Daudi cells by ICC/IF

  • Immunofluorescence of BTK in Daudi cells using ab25971 at 10 ug/ml.

References for Anti-BTK antibody (ab25971)

ab25971 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

I am sorry to confirm thatBTK antibody (ab25971) has not specifically been tested for cross reactivity with the ITK protein.

We ran a BLAST with the immunogen and ITK did not come up with any alignment....

Read More

Thank you for contacting us and your interest in our products.

Through performing a blast search using the immunogen used to raise the anti-BTK antibody (ab25971) no ITK proteins were identified. To check this result, a direct comparison with...

Read More