Anti-BTK (phospho Y223) antibody [EP420Y] (ab68217)


  • Product nameAnti-BTK (phospho Y223) antibody [EP420Y]
    See all BTK primary antibodies
  • Description
    Rabbit monoclonal [EP420Y] to BTK (phospho Y223)
  • SpecificityThis antibody detects BTK phosphorylated on tyrosine 223.
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human BTK.

  • Positive control
    • Ramos cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.



Our Abpromise guarantee covers the use of ab68217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).

For unpurified, use 1/10000 - 1/50000.

IP 1/20.

For unpurified, use 1/40.

  • Application notesIs unsuitable for ICC/IF or IHC-P.
  • Target

    • FunctionPlays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
    • Involvement in diseaseDefects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
      Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
      Contains 1 Btk-type zinc finger.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • Post-translational
      Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
    • Cellular localizationCytoplasm. Membrane. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Agammaglobulinaemia tyrosine kinase antibody
      • AGMX 1 antibody
      • AGMX1 antibody
      • AT antibody
      • ATK antibody
      • B cell progenitor kinase antibody
      • B-cell progenitor kinase antibody
      • BPK antibody
      • Bruton agammaglobulinemia tyrosine kinase antibody
      • Bruton tyrosine kinase antibody
      • Bruton’s Tyrosine Kinase antibody
      • Btk antibody
      • BTK_HUMAN antibody
      • dominant-negative kinase-deficient Brutons tyrosine kinase antibody
      • IMD 1 antibody
      • IMD1 antibody
      • MGC126261 antibody
      • MGC126262 antibody
      • OTTHUMP00000063593 antibody
      • PSCTK 1 antibody
      • PSCTK1 antibody
      • truncated Bruton agammaglobulinemia tyrosine kinase antibody
      • Tyrosine protein kinase BTK antibody
      • Tyrosine-protein kinase BTK antibody
      • tyrosine-protein kinase BTK isoform (lacking exon 14 antibody
      • XLA antibody
      see all

    Anti-BTK (phospho Y223) antibody [EP420Y] images

    • All lanes : Anti-BTK (phospho Y223) antibody [EP420Y] (ab68217) at 1/10000 dilution (purified)

      Lane 1 : Untreated K562 cell lysate
      Lane 2 : K562 cells treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 76 kDa
      Observed band size : 76 kDa
    • ab68217 (purified) at 1/50 immunoprecipitating BTK (phospho Y223) in Ramos cells (Lane 1) treated with 1 mM Pervanadate for 30 min. For western blotting, a HRP-conjugated anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-BTK (phospho Y223) antibody [EP420Y] (ab68217) at 1/500000 dilution (unpurified)

      Lane 1 : Ramos cell lysates, cells un-treated
      Lane 2 : Ramos cell lysates, cells treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 76 kDa
      Observed band size : 76 kDa

    References for Anti-BTK (phospho Y223) antibody [EP420Y] (ab68217)

    This product has been referenced in:
    • Guo W  et al. Targeting Btk/Etk of prostate cancer cells by a novel dual inhibitor. Cell Death Dis 5:e1409 (2014). Read more (PubMed: 25188519) »
    • Dong S  et al. IPI-145 antagonizes intrinsic and extrinsic survival signals in chronic lymphocytic leukemia cells. Blood 124:3583-6 (2014). WB ; Human . Read more (PubMed: 25258342) »

    See all 5 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application ELISA
    Sample Human Cell (Ramos cells)
    Specification Ramos cells
    Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1.0% · Temperature: 37°C
    Type Sandwich (Detection)

    Biao Xi

    Verified customer

    Submitted Oct 07 2013

    Thank you very much for your interest in ab68217.

    To our knowledge, ab68217 has not been tested in flow cytometry. Therefore, I can offer a discount off a future purchase if you buy ab68217 now, test it in flow cytometry and submit feedback ...

    Read More