Recombinant
RabMAb

Anti-BTN1A1 antibody [EPR14173] (ab180605)

Overview

  • Product name
    Anti-BTN1A1 antibody [EPR14173]
    See all BTN1A1 primary antibodies
  • Description
    Rabbit monoclonal [EPR14173] to BTN1A1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human BTN1A1 aa 50-150. The exact sequence is proprietary.
    Database link: Q13410

  • Positive control
    • Human milk.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR14173
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab180605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 62, 66 kDa (predicted molecular weight: 59 kDa).
IP 1/50.

Target

  • Function
    May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane (By similarity). Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 B30.2/SPRY domain.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Membrane. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BT antibody
    • BT1A1_HUMAN antibody
    • BTN antibody
    • BTN1 antibody
    • Btn1a1 antibody
    • butyrophilin antibody
    • Butyrophilin subfamily 1 member A1 antibody
    • butyrophilin, subfamily 1, member A1 antibody
    see all

Images

  • Anti-BTN1A1 antibody [EPR14173] (ab180605) at 1/50000 dilution + Human milk at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 59 kDa
    Observed band size : 62,66 kDa (why is the actual band size different from the predicted?)

    66 kDa: full length
    62 kDa: fragment

  • Western blot analysis of Human milk  immunoprecipitated with ab180605 at 1/50 dilution (Lane 1). Lane2 : Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG, used at 1/1500 dilution.

References

ab180605 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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