Overview

  • Product name
  • Description
    Rabbit polyclonal to BTN2A1
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: ILSGEKEFER ETREIALKEL EKERVQKEEE LQVKEKLQEE LRWRRTFLHA VDVVLD, corresponding to amino acids 278-333 of Human BTN2A1.

  • Positive control
    • Human skeletal muscle tissue; RT 4 and U 251 MG lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab121593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Tissue specificity
    Highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas. Moderate expression was seen in lung, liver and kidney.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 B30.2/SPRY domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BK14H9.1 antibody
    • BT2.1 antibody
    • BT2A1_HUMAN antibody
    • BTF1 antibody
    • BTN2A1 antibody
    • Butyrophilin BTF1 antibody
    • Butyrophilin subfamily 2 member A1 antibody
    • CTA-14H9.1 antibody
    • DJ3E1.1 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane. Recommended concentration of ab121593 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121593 at 1/50 dilution staining BTN2A1 in Paraffin-embedded Human Skeletal tissue by Immunohistochemistry.
  • All lanes : Anti-BTN2A1 antibody (ab121593) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

References

ab121593 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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