Overview

  • Product nameAnti-Bu1a antibody [21-1A4]
    See all Bu1a primary antibodies
  • Description
    Mouse monoclonal [21-1A4] to Bu1a
  • Specificityab25373 recognises Bu1a on bursal cells, thymocytes, spleen cells and peripheral blood cells. It does not react with cells from CHA and H.B15 strains by immunofluorescence.
  • Tested applicationsSuitable for: Flow Cyt, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Tissue/ cell preparation: Bursal cells from one day old H.B15 (Bu-1a/b) strain chickens.

  • Positive control
    • Chicken peripheral blood mononuclear cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • ClonalityMonoclonal
  • Clone number21-1A4
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceBu1a, a product of the Bu1a allele, is expressed on the cell surface as a disulphide linked homodimer.
  • Cellular localizationCell membrane.
  • Database links
  • Alternative names
    • B6.1 antibody
    • Bu 1a antibody
    • Bu1a antibody

Anti-Bu1a antibody [21-1A4] images

  • Chicken peripheral blood mononuclear cells were first incubated with ab25373 (1 µg/106 cells) and then counter stained with goat anti mouse IgG FITC. Small lymphocytes were gated using light scatter criteria and analyzed on a flow cytometer.

References for Anti-Bu1a antibody [21-1A4] (ab25373)

This product has been referenced in:

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"