Anti-Bu1a antibody [21-1A4] (Phycoerythrin) (ab25433)

Overview

  • Product nameAnti-Bu1a antibody [21-1A4] (Phycoerythrin)
    See all Bu1a primary antibodies
  • Description
    Mouse monoclonal [21-1A4] to Bu1a (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityChicken Bu1a alloantigen, Mr 70 kDa but does not react with cells from CHA and H.B15 strains by immunofluorescence.
  • Tested applicationsSuitable for: Flow Cyt, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Bursal cells from one day old H.B15 (Bu-1a/b) strain chickens

  • Positive control
    • Chicken peripheral blood mononuclear cells
  • General notesTo insure consistent performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS; Stabilizing agent
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number21-1A4
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
IP Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

Target

  • RelevanceBu1a, a product of the Bu1a allele, is expressed on the cell surface as a disulphide linked homodimer.
  • Cellular localizationCell membrane.
  • Database links
  • Alternative names
    • B6.1 antibody
    • Bu 1a antibody
    • Bu1a antibody

Anti-Bu1a antibody [21-1A4] (Phycoerythrin) images

  • Flow cytometry analysis staining Bu1a in chicken peripheral blood mononuclear cells using ab25433 at a dilution of 0.1 μg/106 cells.

References for Anti-Bu1a antibody [21-1A4] (Phycoerythrin) (ab25433)

ab25433 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"