The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration. Fix with acetone
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceThe Bu1 [chB6] molecule is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has long been used as an allotypic marker in important studies of B cell development, though its function is unknown. It has no recognizable similarity to known mammalian molecules and thus represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. The sequences of the different alleles of this gene revealed a higher level of polymorphism than expected. A restriction fragment length polymorphism linked to the CHB6 gene has been used to determine its location on the linkage map of the chicken genome, which will allow the definitive evaluation of reported associations with disease resistance.
Bu1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
References for Anti-Bu1a + Bu1b antibody [AV20] (ab25400)
This product has been referenced in:
Bradaric MJ et al. Immune Cells in the Normal Ovary and Spontaneous Ovarian Tumors in the Laying Hen (Gallus domesticus) Model of Human Ovarian Cancer. PLoS One8:e74147 (2013).
IHC-P, Flow Cyt
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Rothwell CJ et al. Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20. Vet Immunol Immunopathol55:225-34 (1996).
Read more (PubMed: 9014319) »