Anti-Bu1a + Bu1b antibody [AV20] (FITC) (ab24901)

Overview

  • Product nameAnti-Bu1a + Bu1b antibody [AV20] (FITC)
    See all Bu1a + Bu1b primary antibodies
  • Description
    Mouse monoclonal [AV20] to Bu1a + Bu1b (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cyt, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Chicken
    Does not react with: Turkey
  • Immunogen

    Tissue/ cell preparation: Bursal cells from one day old H.B15 (Bu1a/b) strain chickens.

Properties

Applications

Our Abpromise guarantee covers the use of ab24901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceThe Bu1 [chB6] molecule is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has long been used as an allotypic marker in important studies of B cell development, though its function is unknown. It has no recognizable similarity to known mammalian molecules and thus represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. The sequences of the different alleles of this gene revealed a higher level of polymorphism than expected. A restriction fragment length polymorphism linked to the CHB6 gene has been used to determine its location on the linkage map of the chicken genome, which will allow the definitive evaluation of reported associations with disease resistance. Bu1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
  • Cellular localizationType I membrane protein.
  • Database links
  • Alternative names
    • B6.1 antibody
    • B6.2 antibody
    • Bu 1a antibody
    • Bu 1b antibody
    • LOC396098 antibody
    see all

Anti-Bu1a + Bu1b antibody [AV20] (FITC) images

  • Chicken peripheral blood mononuclear cells were double stained with mouse anti-chicken CD3-R-PE (ab25419) and ab24901 (1 ug/106 cells). Lymphocytes were then gated and the cells analyzed on a FACScan flow cytometer (BDIS, San Jose, CA).

References for Anti-Bu1a + Bu1b antibody [AV20] (FITC) (ab24901)

ab24901 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Chicken Cell (Lymphocytes)
Specification Lymphocytes
Fixation Formaldehyde
Permeabilization No
Gating Strategy B-cells
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Submitted Dec 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"