Anti-Bu1a + Bu1b antibody [AV20] (Phycoerythrin) (ab25437)

Overview

  • Product nameAnti-Bu1a + Bu1b antibody [AV20] (Phycoerythrin)
    See all Bu1a + Bu1b primary antibodies
  • Description
    Mouse monoclonal [AV20] to Bu1a + Bu1b (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab25437 recognises Bu1a and Bu1b.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Chicken
    Does not react with: Turkey
  • Immunogen

    Tissue/ cell preparation: Bursal cells from one day old H.B15 (Bu 1a/b) strain chickens.

Properties

Applications

Our Abpromise guarantee covers the use of ab25437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IHC-Fr
IP
  • Application notesFlow Cyt: Use 0.2µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    Note: samples need to be acetone fixed.
    IP: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceThe Bu1 [chB6] molecule is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has long been used as an allotypic marker in important studies of B cell development, though its function is unknown. It has no recognizable similarity to known mammalian molecules and thus represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. The sequences of the different alleles of this gene revealed a higher level of polymorphism than expected. A restriction fragment length polymorphism linked to the CHB6 gene has been used to determine its location on the linkage map of the chicken genome, which will allow the definitive evaluation of reported associations with disease resistance. Bu1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
    • Cellular localizationType I membrane protein.
    • Database links
    • Alternative names
      • B6.1 antibody
      • B6.2 antibody
      • Bu 1a antibody
      • Bu 1b antibody
      • LOC396098 antibody
      see all

    References for Anti-Bu1a + Bu1b antibody [AV20] (Phycoerythrin) (ab25437)

    This product has been referenced in:
    • Rothwell CJ  et al. Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20. Vet Immunol Immunopathol 55:225-34 (1996). Read more (PubMed: 9014319) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab25437.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"