Overview

  • Product nameAnti-Bu1b antibody [5-11G2]
    See all Bu1b primary antibodies
  • Description
    Mouse monoclonal [5-11G2] to Bu1b
  • Specificityab25391 recognises Bu1b on bursal cells, thymocytes, spleen cells and peripheral blood cells. It does not react with cells from CHA and H.B15Ab strains by immunofluorescence.
  • Tested applicationsSuitable for: Flow Cyt, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Tissue/ cell preparation: Bursal cells from 2 to 5 week old CHA (Bu1b) strain chickens.

  • Positive control
    • Chicken peripheral blood mononuclear cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 8.2
    Constituent: 100% Borate buffered saline

    No preservatives or amine-containing buffer salts added
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number5-11G2
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceBu1b is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has no recognizable similarity to known mammalian molecules and therefore represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. Chicken Bu1b, a product of the Bu1b allele, is expressed on the cell surface as a disulfide linked homodimer. In 10 week old H.B15 and H.B2 chickens, Bu1 is found on 85 to 90% of bursal cells, 2 to 8% of thymocytes, 15 to 27% of spleen cells, and 2 to 18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu1 alleles (Bu1 a/b), Bu1a does not exhibit allelic exclusion.
  • Cellular localizationCell membrane
  • Database links
  • Alternative names
    • B6.1 antibody
    • B6.2 antibody
    • Bu 1b antibody
    • LOC396098 antibody

Anti-Bu1b antibody [5-11G2] images

  • Chicken peripheral blood mononuclear cells were first incubated with ab25391 (1 µg/106 cells) and then counter stained with a FITC-conjugated goat anti mouse IgG antibody. Small lymphocytes were gated using lignt scatter criteria and analyzed on a flow cytometer.

References for Anti-Bu1b antibody [5-11G2] (ab25391)

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"