Anti-Bu1b antibody [5-11G2] (Phycoerythrin) (ab25434)

Overview

  • Product nameAnti-Bu1b antibody [5-11G2] (Phycoerythrin)
    See all Bu1b primary antibodies
  • Description
    Mouse monoclonal [5-11G2] to Bu1b (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityThis antibody is specific to Chicken Bu1b alloantigen, Mr 70 kDa. The antibody does not react with cells from CHA and H.B15Ab strains by immunofluorescence.
  • Tested applicationsSuitable for: Flow Cyt, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Tissue/ cell preparation: Bursal cells from 2 to 5 week old CHA (Bu1b) strain chickens.

  • Positive control
    • Chicken peripheral blood mononuclear cells for Immunofluorescent staining and flow cytometric analysis.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS; Stabilizing agent
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number5-11G2
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IP
IHC-Fr
  • Application notesFlow Cyt: Use up to 0.2µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceBu1b is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has no recognizable similarity to known mammalian molecules and therefore represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. Chicken Bu1b, a product of the Bu1b allele, is expressed on the cell surface as a disulfide linked homodimer. In 10 week old H.B15 and H.B2 chickens, Bu1 is found on 85 to 90% of bursal cells, 2 to 8% of thymocytes, 15 to 27% of spleen cells, and 2 to 18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu1 alleles (Bu1 a/b), Bu1a does not exhibit allelic exclusion.
    • Cellular localizationCell membrane
    • Database links
    • Alternative names
      • B6.1 antibody
      • B6.2 antibody
      • Bu 1b antibody
      • LOC396098 antibody

    References for Anti-Bu1b antibody [5-11G2] (Phycoerythrin) (ab25434)

    ab25434 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"