Overview

  • Product nameAnti-Bub1 antibody
    See all Bub1 primary antibodies
  • Description
    Rabbit polyclonal to Bub1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Fusion protein (His-tag) corresponding to Human Bub1 aa 1-312 (N terminal).
    Database link: O43683

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/2000. Predicted molecular weight: 122 kDa.

Target

  • FunctionSerine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.
  • Tissue specificityHigh expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.
    Contains 1 BUB1 N-terminal domain.
    Contains 1 protein kinase domain.
  • DomainThe KEN box is required for its ubiquitination and degradation.
    BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR. Upon spindle-assembly checkpoint activation it is hyperphosphorylated and its kinase activity toward CDC20 is stimulated. Phosphorylation at Thr-609 is required for interaction with PLK1, phosphorylation at this site probably creates a binding site for the POLO-box domain of PLK1, thus enhancing the PLK1-BUB1 interaction.
    Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, CASC5 and INCENP are required for kinetochore localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bub1 antibody
    • BUB1 budding uninhibited by benzimidazoles 1 homolog antibody
    • BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody
    • BUB1 mitotic checkpoint serine/threonine kinase antibody
    • BUB1, S. cerevisiae, homolog of antibody
    • BUB1_HUMAN antibody
    • BUB1A antibody
    • BUB1L antibody
    • Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody
    • Budding uninhibited by benzimidazoles 1 homolog antibody
    • Budding uninhibited by benzimidazoles 1, S. cerevisiae, homolog of antibody
    • hBUB1 antibody
    • Homolog of mitotic checkpoint gene BUB1 antibody
    • Mitotic checkpoint gene BUB1 antibody
    • Mitotic checkpoint serine/threonine protein kinase BUB1 antibody
    • Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody
    • Mitotic spindle checkpoint kinase antibody
    • Putative serine/threonine protein kinase antibody
    see all

Anti-Bub1 antibody images



  • Predicted band size : 122 kDa


    Western blot using ab9000 on Bub1 antigen (lanes 1- 3), and HL-60 whole cell extract.

    The Bub1 antigen is only a fragment of full length Bub1 and thus runs at a lower size than the band seen in HL-60 extract.

  • ab9000 staining Bub1 in HCT cells by Immunocytochemistry/ Immunofluorescence. Cells were plated on 12 mm coverslips, pre-extracted with 0.2% Triton X-100 in warm PEM (100 mM PIPES pH6.8, 1mM MgCl2 and 5mM EGTA) for 1 minute, fixed with 3% PFA in PBS, blocked with 3% BSA in PBS for 1 hour, incubated with primary antibody for 16 hrs at 4°C, washed with PBS/0.1% Triton X-100 and incubated with secondary antibody for an additional 1 hour at room temperature. ACA staining in red, Bub1 is green and DNA (DAPI) is in blue.
  • ab9000, staining Bub1 in oocytes from Sprague-Dawley rats.

    Oocytes were fixed in paraformaldehyde, permeabilized with 0.5% Triton-X 100 in PHEM and blocked in PHEM containing 3% BSA for 1 hour. Samples were incubated with primary antibody at 1/500 dilution overnight at 4°C. A Cy3-conjugated goat-anti-rabbit IgG (1/800) was used as the secondary antibody.

References for Anti-Bub1 antibody (ab9000)

This product has been referenced in:
  • Baron AP  et al. Probing the catalytic functions of Bub1 kinase using the small molecule inhibitors BAY-320 and BAY-524. Elife 5:N/A (2016). WB, IF ; Human . Read more (PubMed: 26885717) »
  • Santaguida S  et al. Aneuploidy-induced cellular stresses limit autophagic degradation. Genes Dev 29:2010-21 (2015). Read more (PubMed: 26404941) »

See all 10 Publications for this product

Product Wall

Thank you for contacting us.

I have attached the MSDS for non-hazardous product for ab55954.

The MSDS for ab9000 can be printed from the online datasheet: http://www.abcam.com/bub1-antibody-ab9000.html
The button is underneath...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"