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Synthetic peptide derived from residues 300 to the C-terminus of Human Bub3.
Our Abpromise guarantee covers the use of ab4180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).|
|EMSA||Use at an assay dependent concentration. PubMed: 22210889|
Western blot of ab4180 on 20ug HeLa Nuclear Extract at 1/500.
Secondary ab: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000 - 1 min exposure)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"