Anti-Bub3 antibody (ab69600)


  • Product nameAnti-Bub3 antibody
    See all Bub3 primary antibodies
  • Description
    Rabbit polyclonal to Bub3
  • SpecificityDetects endogenous levels of total Bub3 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the C-terminus of human Bub3.

  • Positive control
    • Extracts from HeLa, Jurkat and 293 cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab69600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ELISA 1/10000.


  • FunctionHas a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
  • Sequence similaritiesBelongs to the WD repeat BUB3 family.
    Contains 5 WD repeats.
  • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aa2-050 antibody
    • AU019800 antibody
    • AU021329 antibody
    • AU043350 antibody
    • AW146323 antibody
    • BUB 3 antibody
    • BUB 3L antibody
    • BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog antibody
    • bub3 antibody
    • BUB3 budding uninhibited by benzimidazoles 3 homolog antibody
    • BUB3 mitotic checkpoint protein antibody
    • BUB3_HUMAN antibody
    • BUB3L antibody
    • Budding uninhibited by benomyl antibody
    • Budding uninhibited by benzimidazoles 3 antibody
    • Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) antibody
    • Budding uninhibited by benzimidazoles 3 homolog (yeast) antibody
    • Budding uninhibited by benzimidazoles 3 homolog antibody
    • Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody
    • C78067 antibody
    • hBUB3 antibody
    • Mitotic checkpoint component antibody
    • Mitotic checkpoint protein bub3 antibody
    • OTTHUMP00000020679 antibody
    • OTTHUMP00000020680 antibody
    • WD repeat type I transmembrane protein A72.5 antibody
    see all

Anti-Bub3 antibody images

  • All lanes : Anti-Bub3 antibody (ab69600) at 1/500 dilution

    Lane 1 : Extract from HeLa cells
    Lane 2 : Extract from Jurkat cells
    Lane 3 : Extract from 293 cells
    Lane 4 : Extract from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa

References for Anti-Bub3 antibody (ab69600)

ab69600 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Methanol and Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Feb 22 2011

Thank you for your enquiry. Having performed homology searches of the immunogens of our Bub3 antibodies with the Drosophila protein I regret to inform you that neither antibody seem suitable for this species due to low sequence homology. I'm sorr...

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