Overview

  • Product nameAnti-Bub3 antibody
    See all Bub3 primary antibodies
  • Description
    Rabbit polyclonal to Bub3
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 275 and 325 of Human Bub3 (Swiss-Prot O43684).

  • Positive control
    • Human metastatic lymph node. Human prostate adenocarcinoma.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHas a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
    • Sequence similaritiesBelongs to the WD repeat BUB3 family.
      Contains 5 WD repeats.
    • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
    • Information by UniProt
    • Database links
    • Alternative names
      • Aa2-050 antibody
      • AU019800 antibody
      • AU021329 antibody
      • AU043350 antibody
      • AW146323 antibody
      • BUB 3 antibody
      • BUB 3L antibody
      • BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog antibody
      • bub3 antibody
      • BUB3 budding uninhibited by benzimidazoles 3 homolog antibody
      • BUB3 mitotic checkpoint protein antibody
      • BUB3_HUMAN antibody
      • BUB3L antibody
      • Budding uninhibited by benomyl antibody
      • Budding uninhibited by benzimidazoles 3 antibody
      • Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) antibody
      • Budding uninhibited by benzimidazoles 3 homolog (yeast) antibody
      • Budding uninhibited by benzimidazoles 3 homolog antibody
      • Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody
      • C78067 antibody
      • hBUB3 antibody
      • Mitotic checkpoint component antibody
      • Mitotic checkpoint protein bub3 antibody
      • OTTHUMP00000020679 antibody
      • OTTHUMP00000020680 antibody
      • WD repeat type I transmembrane protein A72.5 antibody
      see all

    Anti-Bub3 antibody images

    • ab84448, at 1/250 dilution, staining Bub3 in formalin-fixed, paraffin-embedded human metastatic lymph node tissue by Immunohistochemistry using DAB detection.
    • ab84448, at 1/250 dilution, staining Bub3 in formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue by Immunohistochemistry using DAB detection.

    References for Anti-Bub3 antibody (ab84448)

    ab84448 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab84448.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"