Overview

  • Product name
  • Description
    Rabbit polyclonal to BUD13
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Baboon, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 150-200 of Human BUD13 according to NP_116114.1

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab117823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 71 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Sequence similarities
    Belongs to the CWC26 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bud13 antibody
    • BUD13 homolog (S. cerevisiae) antibody
    • BUD13 homolog antibody
    • BUD13_HUMAN antibody
    • Cwc26 antibody
    • FLJ27090 antibody
    • fSAP71 antibody
    • Functional spliceosome-associated protein 71 antibody
    • MGC13125 antibody
    • OTTHUMP00000042639 antibody
    • OTTHUMP00000042640 antibody
    see all

Images

  • All lanes : Anti-BUD13 antibody (ab117823) at 0.1 µg/ml

    Lane 1 : Whole HeLa cell lysate at 50 µg
    Lane 2 : Whole HeLa cell lysate at 15 µg

    Developed using the ECL technique

    Predicted band size : 71 kDa


    Exposure time : 3 minutes
  • Detection of Human BUD13 by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab117823 at 6 µg/mg of lysate. Subsequent WB detection was done using ab117823 at 1 µg/ml. Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab117823 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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