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- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
- PurityProtein A purified
- Research areas
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1.25 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 17 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
- Sequence similaritiesBelongs to the BUD31 (G10) family.
- Cellular localizationNucleus.
Information by UniProt
- Bud31 antibody
- BUD31 homolog (S. cerevisiae) antibody
- BUD31_HUMAN antibody
Anti-BUD31 antibody images
Western blot - BUD31 antibody (ab66369)
Anti-BUD31 antibody (ab66369) at 1.25 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk / PBS buffer
HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution at 1/50000 dilution
Predicted band size : 17 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.
References for Anti-BUD31 antibody (ab66369)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"