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butyrate response factor 1 protein (Tagged-His Tag) (ab91901)

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Overview

  • Product namebutyrate response factor 1 protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 31-338 of Human butyrate response factor 1 with N terminal His tag; 308 amino acids, 39kDa (33kDa predicted from sequence). UniProt ID = Q07352.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 97 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesAGGCLLDRKAVGTPAGGGFPRRHSVTLPSSKFHQNQLLSS LKGEPAPALSSRDSRFRDR SFSEGGERLLPTQKQPGGG QVNSSRYKTELCRPFEENGACKYGDKCQFAHGIHELRSLT RHPKYKTELCRTFHTIGFCPYGPRCHFIHNAEERRALA GARDLSADRPRLQHSFSFAGF PSAAATAAATGLLDSPT SITPPPILSADDLLGSPTLPDGTNNPFAFSSQELASLFAP SM GLPGGGSPTTFLFRPMSESPHMFDSPPSPQDSLSDQ EGYLSSSSSSHSGSDSPTLDNSR RLPIFSRLSISDD
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91901 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Berg36BRF1butyrate response factor 1
        cMG1ERF1RNF162BTIS11B
      see all
  • RelevanceButyrate response factor 1 is a member of the TIS11 family of early response genes. Family members are induced by various agonists such as the phorbol ester TPA and the polypeptide mitogen EGF. The gene is well conserved across species and has a promoter that contains motifs seen in other early response genes. The encoded protein contains a distinguishing putative zinc finger domain with a repeating Cys-His motif. This putative nuclear transcription factor probably functions in regulating the response to growth factors.
  • Cellular localizationNuclear

    • butyrate response factor 1 protein (Tagged-His Tag) images

    • SDS-PAGE - butyrate response factor 1 protein (Tagged-His Tag) (ab91901)
      SDS-PAGE - butyrate response factor 1 protein (Tagged-His Tag) (ab91901)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for butyrate response factor 1 protein (Tagged-His Tag) (ab91901)

    ab91901 has not yet been referenced specifically in any publications.

    Publishing research using ab91901 ? Please let us know so that we can cite the reference in this datasheet

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