Overview

  • Product nameAnti-BVES antibody
    See all BVES primary antibodies
  • Description
    Mouse monoclonal to BVES
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: YSLNDPTLND KKAKKLEHQL SLCTQISMLE MRNSIASSSD SDDGLHQFLR GTSSMSSLHV SSPHQRASAK MKPIEEGAED DDDVFEPASP NTLKVHQLP, corresponding to amino acids 262-360 of human BVES (NP_009004) with a 26 kDa tag.

  • Positive control
    • IMR-32 cell lysate; recombinant tagged fragment of human BVES.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 41 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionCell adhesion molecule involved in the establishment and/or maintenance of cell integrity. Involved in the formation and regulation of the tight junction (TJ) paracellular permeability barrier in epithelial cells. Plays a role in VAMP3-mediated vesicular transport and recycling of different receptor molecules through its interaction with VAMP3. Plays a role in the regulation of cell shape and movement by modulating the Rho-family GTPase activity through its interaction with ARHGEF25/GEFT. Induces primordial adhesive contact and aggregation of epithelial cells in a Ca(2+)-independent manner. Also involved in striated muscle regeneration and repair and in the regulation of cell spreading.
  • Tissue specificityExpressed in epithelial cells (at protein level). Expressed in fetal and adult heart and skeletal muscle.
  • Sequence similaritiesBelongs to the popeye family.
  • Cellular localizationLateral cell membrane. Cell junction > tight junction. Membrane. Colocalizes with VAMP3 at the cell-cell contact in cardiac and skeletal muscle (By similarity). Its movement from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact. Colocalizes in epithelial cells with OCLN and TJP1 in an apical-lateral position within the z axis. Detected at cell-cell contact but never observed at the free surface of epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Blood vessel epicardial substance antibody
    • Bves antibody
    • hBVES antibody
    • MGC42413 antibody
    • POP 1 antibody
    • POP1 antibody
    • POPD1_HUMAN antibody
    • POPDC 1 antibody
    • POPDC1 antibody
    • Popeye domain containing 1 antibody
    • Popeye domain containing protein 1 antibody
    • Popeye domain-containing protein 1 antibody
    • Popeye protein 1 antibody
    see all

Anti-BVES antibody images

  • Anti-BVES antibody (ab77639) at 1/500 dilution + Recombinant tagged fragment of human BVES (the immunogen) at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 41 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-BVES antibody (ab77639) at 1/500 dilution + IMR-32 cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 41 kDa
    Observed band size : 41 kDa

References for Anti-BVES antibody (ab77639)

ab77639 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Unfortunately we have not tested this antibody in Immunofluorescence, and I am afraid we do not have any other BVES antibody suitable for this technique in our catalogue. The fact that it has not been tested does n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"