Anti-BZW1 antibody (ab85090)

Overview

  • Product name
    Anti-BZW1 antibody
  • Description
    Rabbit polyclonal to BZW1
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A 16 amino acid peptide from near the C terminal residues of Human BZW1 protein (BAD96965).

  • Positive control
    • Lymph nodes, HeLa , HepG2 and MCF7 cell lysate

Properties

  • Form
    Lyophilised:Reconstitute in 200ul sterile H2O.
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/1000. Predicted molecular weight: 48 kDa.
ELISA 1/20000 - 1/80000.
IHC-P 1/50 - 1/200.

Target

  • Function
    Enhances histone H4 gene transcription but does not seem to bind DNA directly.
  • Sequence similarities
    Belongs to the BZW family.
    Contains 1 W2 domain.
  • Developmental stage
    Expressed in day 3 embryo.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basic leucine zipper and W2 domain containing protein 1 antibody
    • Basic leucine zipper and W2 domain-containing protein 1 antibody
    • basic leucine-zipper protein BZAP45 antibody
    • BZAP45 antibody
    • bzw1 antibody
    • BZW1_HUMAN antibody
    • KIAA0005 antibody
    • Nbla10236 antibody
    • Protein Orf antibody
    • putative protein product of Nbla10236 antibody
    see all

Anti-BZW1 antibody images

  • All lanes : Anti-BZW1 antibody (ab85090) at 1/500 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : MCF7 cell lysate


    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • Immunohistochemical analysis of BZW1 expression in paraffin embedded formalin fixed lymph node section using 1/100 ab85090. note cytoplasmic staining.

References for Anti-BZW1 antibody (ab85090)

ab85090 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunoprecipitation
Immuno-precipitation step
Other - Dynabead IgG
Sample
Human Tissue lysate - whole (brain)
Specification
brain
Total protein in input
50 µg
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Verified customer

Submitted Dec 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (neuronal)
Specification
neuronal
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C
Type
Indirect
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Abcam user community

Verified customer

Submitted Sep 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal)
Loading amount
75 µg
Specification
neuronal
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
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Abcam user community

Verified customer

Submitted Sep 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (neuronal)
Loading amount
75 µg
Specification
neuronal
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Username

Abcam user community

Verified customer

Submitted Sep 03 2012

Thank you for your reply.   The antibody, ab85090 has the possibility to recognize BZW2, but the possibility is low. The antigen peptide is 15aa peptide and 9aa are the same in BZW2.   Please let me know if there is anything else I can help you with.

Thank you for your reply. I am contacting the lab to see what the exact immunogen sequence ab85090 is. When I find out I will compare that to the BZW2 sequence and see what the level of homology is. If there is anything else I can do in...

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