The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
Use at an assay dependent dilution.
FunctionCatalyzes the first step in the removal of the two C-4 methyl groups of 4,4-dimethylzymosterol.
PathwaySteroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 3/6.
Involvement in diseaseMicrocephaly, congenital cataract, and psoriasiform dermatitis
Sequence similaritiesBelongs to the sterol desaturase family.
DomainThe histidine box domains may contain the active site and/or be involved in metal ion binding.