Anti-c-Fos antibody [2G9C3] (ab16902)

Testing discouint code for ab16902 in zebrafish

ANSWER:

DISCOUNT CODE: ********
Expiration date: **********

I am very pleased to hear you would like to accept our offer and test ab16902 in Zebrafish. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Zebrafish and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

We are looking for a c-fos antibody that will work in zebrafish whole mount tissue and we need to try a few, so would appreciate if we could receive a sample to test of Anti-c-Fos antibody [2G9C3] (ab16902)

If your antibody works, we will be happy to report so at the ZFIN database so that other zebrafish labs will know to use it. 

ANSWER:

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. However to be eligable for this program the species to be tested on must have an 85% or greater homology with the immunogen sequence. I have gone over each of the c-Fos antibodies in our catalogue and none meet that critera.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

http://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

We are obtaining 2 bands of incorrect size in Western blot with ab16902

Bands at 71 and 100 kDa, not at expected size.

Human cell line samples.

ANSWER:

Thank you for your call today.

Sorry to hear ab16902 is not working well in western blot. As discussed I would suggest trying the following:

- Lysing cells in RIPA buffer to ensure sufficient cellular lysis and protein release from the nucleus. - 5% BSA for blocking and primary/secondary antibody incubations.

I hope it improves the results. If there are still problems, please do let me know and I can replace the product with an alternative.  

I am interested in potentially using anti-cfos antibody AB16902 in a species of fish. I understand that this antibody has not yet been tested in fish. When I compared the epitope sequence to my fish sequence, it shares 88% identity in this region, with amino acids: kveqmtpeeeekrrirrernkqaaa. Do you think this antibody is likely to cross-react with c-fos protein with this sequence?

ANSWER:

Thank you for your enquiry. Usually we say that anything over 85% homology does have a good chance of cross-reacting, but because we have not tested this species, we can not guarantee that this antibody would work for that species. If you do purchase the antibody and try it in the fish species, please let us know how it goes. An Abreview, positive or negative, can be worth up to 300 Abpoints, and an image can be worth another 100 Abpoints. These Abpoints can be used towards other purchases, attending conferences, and many other promotions listed on the website.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

I am looking for the blocking peptide for ab16902 antibody. Can I use ab7997 c-fos peptide as a blocking peptide for IHC?

ANSWER:

Thank you for your enquiry. We don't have a blocking peptide available for ab16902. Ab7997 is the blocking peptide for ab7963 (Rabbit polyclonal to c-Fos).

Ab16902 and ab7963 are were raised against different regions of Human c-Fos. Ab16902 was raised against the synthetic peptide: KVEQLSPEEEEKRRIRRERNKMAAA, corresponding to Internal sequence amino acids 128 - 152 of Human c-Fos. Ab7963 was raised against the synthetic peptide: MMFSGFNADYEASS, corresponding to N terminal amino acids 1-14 of Human c-Fos.

Please contact us again if you have any additional questions.