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Anti-c-Fos antibody [2G9C3] (ab16902)

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Overview

Product name

Anti-c-Fos antibody [2G9C3]
See all c-Fos products (22) ...

Description

Mouse monoclonal [2G9C3] to c-Fos

Specificity

Reacts with both cellular and viral forms, will detect both the 55 kDa and 62 kDa c-fos and v-fos proteins, but does not react with the 39 kDa jun protein.

Tested applications

ICC, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide: KVEQLSPEEEEKRRIRRERNKMAAA, corresponding to Internal sequence amino acids 128 - 152 of Human c-Fos.

KVEQLSPEEE EKRRIRRERN KMAAA

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HeLa cells

Properties

Form

Liquid

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, 0.05M Sodium phosphate

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

2G9C3

Isotype

IgG2a

Research areas

Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker

Applications

Show applications key

Our Abpromise guarantee covers the use of ab16902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    ICC

     ICC: Use a concentration...Read more →

    ICC: Use a concentration of 2.5 - 5.0 µg/ml.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 2.5 - 5.0 µg/ml.Detects a band of approximately 55 , 62 kDa (predicted molecular weight: 42 kDa).

Application notes

Is unsuitable for or IHC-P.

Target

Function

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.

Sequence similarities

Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain.

Post-translational
modifications

Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP01100 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Cellular oncogene fos antibody
  • Cellular oncogene fos antibody
  • FBJ murine osteosarcoma viral v fos oncogene homolog antibody
  • FBJ murine osteosarcoma viral v fos oncogene homolog antibody
  • FBJ Osteosarcoma Virus antibody
  • FBJ Osteosarcoma Virus antibody
  • FOS antibody
  • FOS protein antibody
  • FOS_HUMAN antibody
  • G0 G1 switch regulatory protein 7 antibody
  • G0 G1 switch regulatory protein 7 antibody
  • G0/G1 switch regulatory protein 7 antibody
  • G0S7 antibody
  • G0S7 antibody
  • Oncogene FOS antibody
  • Oncogene FOS antibody
  • Proto oncogene protein c fos antibody
  • Proto oncogene protein c fos antibody
  • Proto-oncogene c-Fos antibody
  • v fos FBJ murine osteosarcoma viral oncogene homolog antibody
  • v fos FBJ murine osteosarcoma viral oncogene homolog antibody
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Database links

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References for Anti-c-Fos antibody [2G9C3] (ab16902)

This product has been referenced in:

  • MacNeil LGet al. Eccentric exercise activates novel transcriptional regulation of hypertrophic signaling pathways not affected by hormone changes. PLoS One 5:e10695 (2010). WB; Human.Read more (PubMed: 20502695) »

See 1 publication for this product

Publishing research using ab16902? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"