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Human breast carcinoma tissue labelled with ab53036 at 1/50 dilution; in the presence (+) and absence (-) of the immunizing peptide
All lanes : Anti-c-Fos antibody (ab53036) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with peptide
Predicted band size : 41 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
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