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ab7963 has been referenced in 14 publications.
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c-fos antibody (ab7963) at a dilution of 1/1000 (2 X o/n incubation at RT; ABC amplification). Nuclear staining observed in the rat spinal cord [A] dorsal horn and [B] Lamina 1 of the dorsal horn. Staining also observed in the ventral horn of the spinal cord (not shown). Staining performed in free floating IHC on 30
Image courtesy of Dr S Pezet, CARD institute, KCL, London, UK
Predicted band size : 41 kDa
ab7963 staining c-Fos in A431 whole cell lysate (lane A) and HeLa whole cell lysate (lane B)
c-fos antibody (ab7963) at a dilution of 1/500 (1 day incubation at RT; secondary antibody Alexa fluor 488 at 1/1000). Nuclear staining observed in the rat spinal cord dorsal horn [A] X10 and [B] X20 magnification. Immunofluorescence staining performed on slide on 30
Image courtesy of Dr S Pezet, CARD institute, KCL, London, UK
Overlay histogram showing Jurkat cells stained with ab7963 (blue line). The cells were prepared as follows: Spin down, wash in FACS buffer 1x, fix, wash 2x, and stain with primary antibody overnight. Buffer was 0.1% sodium azide with FBS in phosphate buffered solution. Cells were fixed using paraformaldehyde and permeabilized using Triton X-100 and NP40. Cells were gated by isolating cell population from plot of SSC-A / FSA-A. ab7963 was diluted 1/300 and incubated at 4°C for 24 hours overnight. The secondary antibody used was a FITC-conjugated Goat anti-Rabbit polyclonal, diluted 1/100.
This image is courtesy of an anonymous Abreview.
All lanes : Anti-c-Fos antibody (ab7963) at 1 µg/ml
Lane 1 :
Lane 2 :
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 20 seconds
3
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