If your product does not perform as described on this datasheet, we will refund or replace your product...
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about antibodies to be used in ChIP |
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Thank you very much for your call this morning. |
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IHC (PFA fixed) with mouse brian sections |
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Thank you for contacting us. |
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It would be great if you could refund me the charges for the antibody. |
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Thank you for contacting us. Your credit note ID is I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice. |
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Thank you for sending me a replacement c-FOS antibody immediately. Unfortunately it didn`t work for me either, I compared this antibody (ab7963 Lot# GR71321-2) with the first one you sent me (ab7963 Lot# GR68799-1) and the one that a colleague purchased (ab7963 Lot# GR29485-1) in the same experiment on mouse thoracal spinal cord (which abcam proposes as positive control) under identical conditions. The results of my colleagues antibody were convincing, clear nuclear staining which colocalized with DAPI as expected for c-FOS.However, the first antibody I purchased gave me a cytoplasmatic staining and the replacement antibody didn`t stain anything specific at all, as mentioned, all parallel in the same experiment. |
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Thank you for your message and for providing this further information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
c-fos antibody (ab7963) at a dilution of 1/1000 (2 X o/n incubation at RT; ABC amplification). Nuclear staining observed in the rat spinal cord [A] dorsal horn and [B] Lamina 1 of the dorsal horn. Staining also observed in the ventral horn of the spinal cord (not shown). Staining performed in free floating IHC on 30
Image courtesy of Dr S Pezet, CARD institute, KCL, London, UK
Predicted band size : 41 kDa
ab7963 staining c-Fos in A431 whole cell lysate (lane A) and HeLa whole cell lysate (lane B)
c-fos antibody (ab7963) at a dilution of 1/500 (1 day incubation at RT; secondary antibody Alexa fluor 488 at 1/1000). Nuclear staining observed in the rat spinal cord dorsal horn [A] X10 and [B] X20 magnification. Immunofluorescence staining performed on slide on 30
Image courtesy of Dr S Pezet, CARD institute, KCL, London, UK
Overlay histogram showing Jurkat cells stained with ab7963 (blue line). The cells were prepared as follows: Spin down, wash in FACS buffer 1x, fix, wash 2x, and stain with primary antibody overnight. Buffer was 0.1% sodium azide with FBS in phosphate buffered solution. Cells were fixed using paraformaldehyde and permeabilized using Triton X-100 and NP40. Cells were gated by isolating cell population from plot of SSC-A / FSA-A. ab7963 was diluted 1/300 and incubated at 4°C for 24 hours overnight. The secondary antibody used was a FITC-conjugated Goat anti-Rabbit polyclonal, diluted 1/100.
This image is courtesy of an anonymous Abreview.
All lanes : Anti-c-Fos antibody (ab7963) at 1 µg/ml
Lane 1 :
Lane 2 :
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 20 seconds
3
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