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Immunohistochemistry (PFA perfusion fixed frozen sections) - c-Fos antibody (ab7963)
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- c-Fos antibody (ab7963)
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Immunohistochemistry (PFA perfusion fixed frozen sections) - c-Fos antibody (ab7963)
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Product Name
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c-Fos antibody
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See all c-Fos antibodies (20)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to c-Fos
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Immunogen
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Synthetic peptide: MMFSGFNADYEASS, corresponding to N terminal amino acids 1-14 of Human c-Fos(Peptide available as ab7997.)
BLAST 'MMFSGFNADYEASS' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Reacts with c-Fos
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Tested applications
(see key)
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IHC (PFA fixed), IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol (see attached protocol).
Immunohistochemistry (PFA fixed): 1/500 (IF detection) - 1/1000. PFA/picric acid perfusion fixed tissue; 2 days tissue post-fixation (see attached protocol).
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent concentration. Predicted molecular weight: 60 kDa. Can be blocked with c-Fos (N-term) peptide (human).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: A-431 and HeLa lysate
IHC: rat spinal cord
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
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Relevance
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The Fos gene family consists of 4 members: Fos, FosB, FosL1, and FosL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP1. As such, the Fos proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the Fos gene has also been associated with apoptotic cell death. The human oncogene c-Fos is homologous to the Finkel-Biskis-Jinkins (FBJ) murine osteosarcoma virus oncogene. Fos was the first transcription factor identified that has a critical function in regulating the development of cells destined to form and maintain the skeleton.
Fos protein is routinely used as a marker of neuronal activation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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PBS with 0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this c-Fos antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for c-Fos antibody images, references, products related to ab7963 and other tools.
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c-Fos antibody images:
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 Immunohistochemistry (PFA perfusion fixed frozen sections) - c-Fos antibody (ab7963)
c-fos antibody (ab7963) at a dilution of 1/1000 (2 X o/n incubation at RT; ABC amplification). Nuclear staining observed in the rat spinal cord [A] dorsal horn and [B] Lamina 1 of the dorsal horn. Staining also observed in the ventral horn of the spinal cord (not shown). Staining performed in free floating IHC on 30µm saggital spinal cord sections (perfusion fixed tissue). Find detailed info regarding the protocol in the Specific Protocol link. Image courtesy of Dr S Pezet, CARD institute, KCL, London, UK
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- c-Fos antibody (ab7963)
Predicted band size : 60 kDa
ab7963 staining c-Fos in A431 whole cell lysate (lane A) and HeLa whole cell lysate (lane B)
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Immunohistochemistry (PFA perfusion fixed frozen sections) - c-Fos antibody (ab7963)
c-fos antibody (ab7963) at a dilution of 1/500 (1 day incubation at RT; secondary antibody Alexa fluor 488 at 1/1000). Nuclear staining observed in the rat spinal cord dorsal horn [A] X10 and [B] X20 magnification. Immunofluorescence staining performed on slide on 30µm saggital sections of spinal cord (PFA/picric acid perfusion fixed tissue; 2 days tissue post-fixation). Image courtesy of Dr S Pezet, CARD institute, KCL, London, UK
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References for c-Fos antibody (ab7963)
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This product has been used in: (two most recent references)
Ohira K et al. Ischemia-induced neurogenesis of neocortical layer 1 progenitor cells. Nat Neurosci 13:173-9 (2010). PubMed: 20037576
Raskin K et al. Conditional inactivation of androgen receptor gene in the nervous system: effects on male behavioral and neuroendocrine responses. J Neurosci 29:4461-70 (2009). IHC-P; Mouse. PubMed: 19357272
See all 8 publication references for this product.
If you publish research using ab7963 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to c-Fos
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c-Fos antibody - more information
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Customer reviews (feedback) regarding c-Fos antibody |
Customer FAQs regarding c-Fos antibody |
Protocols for c-Fos antibody |
Price and availability of products related to c-Fos antibody |
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c-Fos antibody - related products:
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c-Fos antibody - other tools:
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Contact Abcam with a Technical Enquiry about c-Fos antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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