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Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Overview
Product name
Anti-c-Fos antibody
See all c-Fos products (22) ...
Description
Rabbit polyclonal to c-Fos
Specificity
Reacts with c-Fos
Tested applications
IP, IHC-P, WB, IHC (PFA fixed), Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: MMFSGFNADYEASS, corresponding to N terminal amino acids 1-14 of Human c-Fos(Peptide available as ab7997.)
MMFSGFNADY EASS
Positive control
WB: A-431 and HeLa lysate IHC: rat spinal cord
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
PBS with 0.1% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
IHC-P: 1/100Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB: Use at an assay dependent dilution. Predicted molecular weight: 41 kDa.(Can be blocked with ab7997 c-Fos (N-term) peptide.)
IHC (PFA fixed): 1/500 - 1/1000.(1/500 (IF detection) - 1/1000. PFA/picric acid perfusion fixed tissue; 2 days tissue post-fixation.)
Flow Cyt: 1/300
Target
Function
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
Sequence similarities
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain.
Post-translational
modifications
Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP01100
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cellular oncogene fos antibody
- FBJ murine osteosarcoma viral v fos oncogene homolog antibody
- FBJ Osteosarcoma Virus antibody
see all
Database links
- Entrez Gene: 2353 Human
- Entrez Gene: 14281 Mouse
- Entrez Gene: 314322 Rat
- Omim: 164810 Human
- SwissProt: P01100 Human
- SwissProt: P01101 Mouse
- SwissProt: P12841 Rat
- Unigene: 246513 Mouse
see all
Anti-c-Fos antibody images:
Immunohistochemistry (PFA perfusion fixed frozen sections) - c-Fos antibody (ab7963)
c-fos antibody (ab7963) at a dilution of 1/1000 (2 X o/n incubation at RT; ABC amplification). Nuclear staining observed in the rat spinal cord [A] dorsal horn and [B] Lamina 1 of the dorsal horn. Staining also observed in the ventral horn of the spinal cord (not shown). Staining performed in free floating IHC on 30
Image courtesy of Dr S Pezet, CARD institute, KCL, London, UK
Western blot - Anti-c-Fos antibody (ab7963)
Predicted band size : 41 kDa
ab7963 staining c-Fos in A431 whole cell lysate (lane A) and HeLa whole cell lysate (lane B)
Immunohistochemistry (PFA perfusion fixed frozen sections) - c-Fos antibody (ab7963)
c-fos antibody (ab7963) at a dilution of 1/500 (1 day incubation at RT; secondary antibody Alexa fluor 488 at 1/1000). Nuclear staining observed in the rat spinal cord dorsal horn [A] X10 and [B] X20 magnification. Immunofluorescence staining performed on slide on 30
Image courtesy of Dr S Pezet, CARD institute, KCL, London, UK
Flow Cytometry - Anti-c-Fos antibody (ab7963)
Overlay histogram showing Jurkat cells stained with ab7963 (blue line). The cells were prepared as follows: Spin down, wash in FACS buffer 1x, fix, wash 2x, and stain with primary antibody overnight. Buffer was 0.1% sodium azide with FBS in phosphate buffered solution. Cells were fixed using paraformaldehyde and permeabilized using Triton X-100 and NP40. Cells were gated by isolating cell population from plot of SSC-A / FSA-A. ab7963 was diluted 1/300 and incubated at 4°C for 24 hours overnight. The secondary antibody used was a FITC-conjugated Goat anti-Rabbit polyclonal, diluted 1/100.
This image is courtesy of an anonymous Abreview.
References for Anti-c-Fos antibody (ab7963)
This product has been referenced in:
- Valero Jet al. Short-Term Environmental Enrichment Rescues Adult Neurogenesis and Memory Deficits in APP(Sw,Ind) Transgenic Mice. PLoS One 6:e16832 (2011). IHC-FoFr; Mouse.Read more (PubMed: 21347380) »
- Bonner JFet al. Grafted neural progenitors integrate and restore synaptic connectivity across the injured spinal cord. J Neurosci 31:4675-86 (2011).Read more (PubMed: 21430166) »
See all 14 publications for this product
Publishing research using ab7963? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"