Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
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ab17933 has been referenced in 4 publications.
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Predicted band size : 41 kDa
Lysates prepared from SKBR3 cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with c-Fos [pT232] antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.
The data show that only the peptide corresponding to c-Fos [pT232] blocks the signal, thereby demonstrating the specificity of the antibody. While c-Fos [pT232] is phosphorylated in the basal state of SKBR3, EGF treatment induced an increase in the phosphorylation signal (data not shown).
ab17933 detecting nuclear staining of phosphorylated T232 c-Fos (green colour) in [A] unstimulated cells and [B] NGF stimulated (100 ng/ml for 2 min) adult dorsal root ganglion (DRG) cells cultured for 2 days. ab17933 was used at 1/300 (incubated for 2h at room temperature). Secondary antibody used is anti-rabbit alexa fluor 488 (at 1/1000 for 1hr at room temperature). The cytoplasm of these DRG cells are immunofluorescent for the neuronal marker beta III tubulin (orange colour). The cell diameter is approx. 25
Image courtesy of Dr S Pezet, CARD institute, KCL, London
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