Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
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ab27793 has been referenced in 3 publications.
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All lanes : Anti-c-Fos (phospho T325) antibody (ab27793) at 1/1000 dilution (diluted in a 3% Milk TBST buffer.)
Lane 1 : Non EGF treated A431 cell lysate
Lane 2 : EGF treated A431 cell lysate
Lane 3 : EGF treated A431 cell lysate treated with Lambda phosphatase
Secondary
goat F(ab’)2 anti rabbit IgG HRP conjugate
Predicted band size : 41 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
The figure shows that the phosphorylation of cFos on threonine 325 is induced by EGF treatment and that Lambda phosphatase treatment eliminates the signal, thereby demonstrating the phospho specificity of ab27793.
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