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Products:Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Overview
Product name
Anti-c-Fos (phospho T325) antibody
See all c-Fos products (22) ...
Description
Rabbit polyclonal to c-Fos (phospho T325)
Specificity
ab27793 recognises the cFos phosphorilated at threonine 325 form.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from the region of human cFos that contains threonine 325.
Positive control
Human epidermoid carcinoma (A431) cells stimulated with EGF or 15% serum.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab27793 was negatively preadsorbed using a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non phosphorylated cFos. Immunogen affinity purification followed.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
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Western blot - c-Fos (phospho T325) antibody (ab27793)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Predicted molecular weight: 41 kDa.
IP: Use at an assay dependent dilution. (PubMed: 20410304)
Target
Function
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
Sequence similarities
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain.
Post-translational
modifications
Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP01100
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cellular oncogene fos antibody
- Cellular oncogene fos antibody
- FBJ murine osteosarcoma viral v fos oncogene homolog antibody
see all
Database links
- Entrez Gene: 2353 Human
- Entrez Gene: 14281 Mouse
- Entrez Gene: 314322 Rat
- Omim: 164810 Human
- SwissProt: P01100 Human
- SwissProt: P01101 Mouse
- SwissProt: P12841 Rat
- Unigene: 246513 Mouse
see all
Anti-c-Fos (phospho T325) antibody images:
Western blot - c-Fos (phospho T325) antibody (ab27793)
All lanes : Anti-c-Fos (phospho T325) antibody (ab27793) at 1/1000 dilution (diluted in a 3% Milk TBST buffer.)
Lane 1 : Non EGF treated A431 cell lysate
Lane 2 : EGF treated A431 cell lysate
Lane 3 : EGF treated A431 cell lysate treated with Lambda phosphatase
Secondary
goat F(ab’)2 anti rabbit IgG HRP conjugate
Predicted band size : 41 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
The figure shows that the phosphorylation of cFos on threonine 325 is induced by EGF treatment and that Lambda phosphatase treatment eliminates the signal, thereby demonstrating the phospho specificity of ab27793.
References for Anti-c-Fos (phospho T325) antibody (ab27793)
This product has been referenced in:
- Nakakuki Tet al. Ligand-specific c-Fos expression emerges from the spatiotemporal control of ErbB network dynamics. Cell 141:884-96 (2010). WB; Human.Read more (PubMed: 20493519) »
- Asim Met al. Helicobacter pylori induces ERK-dependent formation of a phospho-c-Fos c-Jun activator protein-1 complex that causes apoptosis in macrophages. J Biol Chem 285:20343-57 (2010). WB, IP; Mouse.Read more (PubMed: 20410304) »
See all 3 publications for this product
Publishing research using ab27793? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"