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Overview

  • Product nameAnti-c-Jun antibodySee all c-Jun primary antibodies ...
  • Description
    Rabbit polyclonal to c-Jun
  • Tested applicationsWB, ICC/IF, IHC-P, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human c-Jun around the phosphorylation site of threonine 231 (P-Q-TP-V-P).

  • Positive controlHuman testis tissue, extracts from Jurkat cells treated with Paclitaxel (1uM, 60mins) and extracts from HuvEc cells treated with EGF (200ng/ml, 15mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab61764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ICC/IF
IHC-P
ELISA
  • Application notesELISA: 1/5000.
    ICC/IF: 1/500 - 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects several bands including one of approximately 36 kDa (predicted molecular weight: 36 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
    • Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
      Contains 1 bZIP (basic-leucine zipper) domain.
    • Post-translational
      modifications      Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
      Acetylated at Lys-271 by EP300.
    • Cellular localizationNucleus.

      • Target information above from: UniProt accession P05412 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        Activator Protein 1 antibodyAP 1 antibodyAP1 antibody
        cJun antibodyEnhancer Binding Protein AP1 antibodyJun Activation Domain Binding Protein antibodyJUN antibodyJun oncogene antibodyJUN protein antibodyJun proto oncogene antibodyJUN_HUMAN antibodyJUNC antibodyOncogene JUN antibodyp39 antibodyProto oncogene c jun antibodyProto oncogene cJun antibodyProto-oncogene c-jun antibodyTranscription Factor AP 1 antibodyTranscription factor AP-1 antibodyTranscription Factor AP1 antibodyV jun avian sarcoma virus 17 oncogene homolog antibodyV jun sarcoma virus 17 oncogene homolog (avian) antibodyV jun sarcoma virus 17 oncogene homolog antibodyV-jun avian sarcoma virus 17 oncogene homolog antibodyvJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
      see all

    Anti-c-Jun antibody images

    • Western blot - c-Jun antibody (ab61764)
      Western blot - c-Jun antibody (ab61764)
      All lanes : Anti-c-Jun antibody (ab61764) at 1/500 dilution

      Lane 1 : Extracts from Jurkat cells treated with Paclitaxel (1uM, 60mins)
      Lane 2 : Extracts from HuvEc cells treated with EGF (200ng/ml, 15mins)
      Lane 3 : Extracts from HuvEc cells treated with Paclitaxel (1uM, 60mins)in teh presence of peptide


      Predicted band size : 36 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - c-Jun antibody (ab61764)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - c-Jun antibody (ab61764)
      Human testis tissue stained with ab61764 at 1/50 dilution, with and without the immunising peptide.

    References for Anti-c-Jun antibody (ab61764)

    ab61764 has not yet been referenced specifically in any publications.

    Publishing research using ab61764 ? Please let us know so that we can cite the reference in this datasheet

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