Overview

  • Product nameAnti-c-Jun antibodySee all c-Jun primary antibodies ...
  • Description
    Rabbit polyclonal to c-Jun
  • Tested applicationsWB, ICC/IF, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human c-Jun around the phosphorylation site of threonine 231 (P-Q-TP-V-P).

  • Positive control
    • Human testis tissue, extracts from Jurkat cells treated with Paclitaxel (1uM, 60mins) and extracts from HuvEc cells treated with EGF (200ng/ml, 15mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab61764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-P
ELISA
  • Application notesELISA: 1/5000.
    ICC/IF: 1/500 - 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects several bands including one of approximately 36 kDa (predicted molecular weight: 36 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
    • Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
      Contains 1 bZIP (basic-leucine zipper) domain.
    • Post-translational
      modifications
      Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
      Acetylated at Lys-271 by EP300.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator Protein 1 antibody
      • AP 1 antibody
      • AP1 antibody
      • cJun antibody
      • Enhancer Binding Protein AP1 antibody
      • Jun Activation Domain Binding Protein antibody
      • JUN antibody
      • Jun oncogene antibody
      • JUN protein antibody
      • Jun proto oncogene antibody
      • JUN_HUMAN antibody
      • JUNC antibody
      • Oncogene JUN antibody
      • p39 antibody
      • Proto oncogene c jun antibody
      • Proto oncogene cJun antibody
      • Proto-oncogene c-jun antibody
      • Transcription Factor AP 1 antibody
      • Transcription factor AP-1 antibody
      • Transcription Factor AP1 antibody
      • V jun avian sarcoma virus 17 oncogene homolog antibody
      • V jun sarcoma virus 17 oncogene homolog (avian) antibody
      • V jun sarcoma virus 17 oncogene homolog antibody
      • V-jun avian sarcoma virus 17 oncogene homolog antibody
      • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
      see all

    Anti-c-Jun antibody images

    • All lanes : Anti-c-Jun antibody (ab61764) at 1/500 dilution

      Lane 1 : Extracts from Jurkat cells treated with Paclitaxel (1uM, 60mins)
      Lane 2 : Extracts from HuvEc cells treated with EGF (200ng/ml, 15mins)
      Lane 3 : Extracts from HuvEc cells treated with Paclitaxel (1uM, 60mins)in teh presence of peptide


      Predicted band size : 36 kDa
    • Human testis tissue stained with ab61764 at 1/50 dilution, with and without the immunising peptide.

    References for Anti-c-Jun antibody (ab61764)

    ab61764 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab61764.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"