Human Predicted to work with:
Synthetic peptide corresponding to Human c-Jun. Within the aa range of 201-250
Synthetic non-phosphopeptide derived from human c-Jun around the phosphorylation site of threonine 231 (P-Q-TP-V-P). Database link: P05412
Human testis tissue, extracts from Jurkat cells treated
with Paclitaxel (1uM, 60mins) and extracts from HuvEc cells treated with EGF (200ng/ml, 15mins).
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/5000.
ICC/IF: 1/500 - 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects several bands including one of approximately 36 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Sequence similaritiesBelongs to the bZIP family. Jun subfamily. Contains 1 bZIP (basic-leucine zipper) domain.
Post-translational modificationsUbiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7. Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity. Acetylated at Lys-271 by EP300.
All lanes : Anti-c-Jun antibody (ab61764) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells treated with Paclitaxel (1uM, 60mins) Lane 2 : Extracts from HuvEc cells treated with EGF (200ng/ml, 15mins) Lane 3 : Extracts from HuvEc cells treated with Paclitaxel (1uM, 60mins)in teh presence of peptide