Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Serine 73.
Our Abpromise guarantee covers the use of ab30620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC - Wholemount||1/100.|
|WB||1/500 - 1/1000. Predicted molecular weight: 36 kDa.|
|IHC-P||Use at an assay dependent concentration.|
ab30620 staining 24hpf WT zebrafish embryos by IHC-wholemount. The embryos were dechorionated and fixed with 4% paraformaldehyde (or Dent's fixative) over 48h at 4 °C. Embryos were then washed two times with PBS and two times with MeOH, permeabilized with methanol for 5 minutes and blocked with 10% goat serum in PBS - Triton for 1 hour. Staining with ab30620 at a 1/100 dilution in PBS-10% goat serum- Triton was performed for 22h at 4°C. A goat anti-rabbit Alexa 568 polyclonal antibody at 1/1000 was used as the secondary antibody.