• Product name
    Anti-c-Jun (phospho S73) antibody
    See all c-Jun primary antibodies
  • Description
    Rabbit polyclonal to c-Jun (phospho S73)
  • Host species
  • Specificity
    ab30620 detects endogenous levels of c-Jun only when phosphorylated at Serine 73.
  • Tested applications
    Suitable for: ICC/IF, IHC - Wholemount, WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
  • Immunogen

    Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Serine 73.

  • Positive control
    • Breast carcinoma. extracts from Hela cells treated with UV.



Our Abpromise guarantee covers the use of ab30620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC - Wholemount 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.


  • Function
    Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
  • Sequence similarities
    Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • Post-translational
    Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator protein 1 antibody
    • AP 1 antibody
    • AP1 antibody
    • cJun antibody
    • Enhancer Binding Protein AP1 antibody
    • Jun Activation Domain Binding Protein antibody
    • JUN antibody
    • Jun oncogene antibody
    • JUN protein antibody
    • Jun proto oncogene antibody
    • JUN_HUMAN antibody
    • JUNC antibody
    • Oncogene JUN antibody
    • p39 antibody
    • Proto oncogene c jun antibody
    • Proto oncogene cJun antibody
    • Proto-oncogene c-jun antibody
    • Transcription Factor AP 1 antibody
    • Transcription factor AP-1 antibody
    • Transcription Factor AP1 antibody
    • V jun avian sarcoma virus 17 oncogene homolog antibody
    • V jun sarcoma virus 17 oncogene homolog (avian) antibody
    • V jun sarcoma virus 17 oncogene homolog antibody
    • V-jun avian sarcoma virus 17 oncogene homolog antibody
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
    see all


  • All lanes : Anti-c-Jun (phospho S73) antibody (ab30620) at 1/500 dilution

    Lane 1 : extracts from untreated Hela cells
    Lane 2 : extracts from UV treated Hela cells

    Predicted band size: 36 kDa
    Observed band size: 42 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 Phospho c-Jun(Ser73) Antibody (ab30620). Left: untreated sample. Right: sample preincubated with synthesized phosphopeptide.
  • ab30620 staining 24hpf WT zebrafish embryos by IHC-wholemount. The embryos were dechorionated and fixed with 4% paraformaldehyde (or Dent's fixative) over 48h at 4 °C. Embryos were then washed two times with PBS and two times with MeOH, permeabilized with methanol for 5 minutes and blocked with 10% goat serum in PBS - Triton for 1 hour. Staining with ab30620 at a 1/100 dilution in PBS-10% goat serum- Triton was performed for 22h at 4°C. A goat anti-rabbit Alexa 568 polyclonal antibody at 1/1000 was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab30620 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Suwei D  et al. NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol 8:120 (2015). Human . Read more (PubMed: 26503334) »
  • Grande V  et al. Neuroprotective and anti-inflammatory roles of the phosphatase and tensin homolog deleted on chromosome Ten (PTEN) Inhibition in a Mouse Model of Temporal Lobe Epilepsy. PLoS One 9:e114554 (2014). IHC ; Mouse . Read more (PubMed: 25501575) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab30620 has never been tested in frozen sections (IHC-Fr). All tested applications covered by the 6 month guarantee are specified on our datasheets, and these are...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
IHC - Wholemount
Zebrafish Embryo (all embryo)
all embryo

Abcam user community

Verified customer

Submitted Mar 14 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Tissue lysate - whole (Brain tumor)
Loading amount
10 µg
Brain tumor
Lysis buffer (urea/thiourea)
Gel Running Conditions
Reduced Denaturing (4-20)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 05 2010


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