Overview

  • Product nameAnti-c-Jun (phospho S73) antibodySee all c-Jun primary antibodies ...
  • Description
    Rabbit polyclonal to c-Jun (phospho S73)
  • Specificityab30620 detects endogenous levels of c-Jun only when phosphorylated at Serine 73.
  • Tested applicationsICC/IF, IHC - Wholemount, WB, IHC-P, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
  • Immunogen

    Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Serine 73.

  • Positive control
    • Breast carcinoma. extracts from Hela cells treated with UV. HepG2 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab30620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC - Wholemount 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
IHC-P Use at an assay dependent dilution.
ELISA 1/10000.

Target

  • FunctionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
  • Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • Post-translational
    modifications
    Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
    Acetylated at Lys-271 by EP300.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession P05412 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Activator Protein 1 antibody
    • AP 1 antibody
    • AP1 antibody
    • cJun antibody
    • Enhancer Binding Protein AP1 antibody
    • Jun Activation Domain Binding Protein antibody
    • JUN antibody
    • Jun oncogene antibody
    • JUN protein antibody
    • Jun proto oncogene antibody
    • JUN_HUMAN antibody
    • JUNC antibody
    • Oncogene JUN antibody
    • p39 antibody
    • Proto oncogene c jun antibody
    • Proto oncogene cJun antibody
    • Proto-oncogene c-jun antibody
    • Transcription Factor AP 1 antibody
    • Transcription factor AP-1 antibody
    • Transcription Factor AP1 antibody
    • V jun avian sarcoma virus 17 oncogene homolog antibody
    • V jun sarcoma virus 17 oncogene homolog (avian) antibody
    • V jun sarcoma virus 17 oncogene homolog antibody
    • V-jun avian sarcoma virus 17 oncogene homolog antibody
    • vJun Avian Sarcoma Virus 17 Oncogene Homolog antibody
    see all

Anti-c-Jun (phospho S73) antibody images

  • All lanes : Anti-c-Jun (phospho S73) antibody (ab30620) at 1/500 dilution

    Lane 1 : extracts from untreated Hela cells
    Lane 2 : extracts from UV treated Hela cells


    Predicted band size : 36 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 Phospho c-Jun(Ser73) Antibody (ab30620). Left: untreated sample. Right: sample preincubated with synthesized phosphopeptide.
  • ab30620 staining 24hpf WT zebrafish embryos by IHC-wholemount. The embryos were dechorionated and fixed with 4% paraformaldehyde (or Dent's fixative) over 48h at 4 °C. Embryos were then washed two times with PBS and two times with MeOH, permeabilized with methanol for 5 minutes and blocked with 10% goat serum in PBS - Triton for 1 hour. Staining with ab30620 at a 1/100 dilution in PBS-10% goat serum- Triton was performed for 22h at 4°C. A goat anti-rabbit Alexa 568 polyclonal antibody at 1/1000 was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab30620 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-c-Jun (phospho S73) antibody (ab30620)

This product has been referenced in:
  • Spigolon G  et al. c-Jun N-terminal kinase signaling pathway in excitotoxic cell death following kainic acid-induced status epilepticus. Eur J Neurosci 31:1261-72 (2010). IHC-P ; Rat . Read more (PubMed: 20345908) »

See 1 Publication for this product

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Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab30620 has never been tested in frozen sections (IHC-Fr). All tested applications covered by the 6 month guarantee are specified on our datasheets, and these are...

Read More
Application IHC - Wholemount
Sample Zebrafish Embryo (all embryo)
Specification all embryo
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Submitted Mar 14 2012

Application Western blot
Sample Human Tissue lysate - whole (Brain tumor)
Loading amount 10 µg
Specification Brain tumor
Treatment Lysis buffer (urea/thiourea)
Gel Running Conditions Reduced Denaturing (4-20)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
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Verified customer

Submitted Feb 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"