Anti-c-Jun (phospho S73) antibody (ab30620)

Overview

• Product nameAnti-c-Jun (phospho S73) antibodySee all c-Jun primary antibodies ...
• Description
  Rabbit polyclonal to c-Jun (phospho S73)
• Specificityab30620 detects endogenous levels of c-Jun only when phosphorylated at Serine 73.
• Tested applicationsICC/IF, IHC - Wholemount, WB, IHC-P, ELISA more details
• Species reactivity
  Reacts with: Mouse, Rat, Human, Zebrafish
  
• Immunogen

  Synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Serine 73.

• Positive controlBreast carcinoma. extracts from Hela cells treated with UV. HepG2 cell line

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide.The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Transcription factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • Leucine Zipper

  • Immunology
  • Innate Immunity
  • TLR Signaling

Applications

Target

• FunctionTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
• Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
  Contains 1 bZIP (basic-leucine zipper) domain.
• Post-translational
  modificationsPhosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
  Acetylated at Lys-271 by EP300.
• Cellular localizationNucleus.

Target information above from: UniProt accession P05412 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3725 Human
  • Entrez Gene: 16476 Mouse
  • Entrez Gene: 24516 Rat
  • Entrez Gene: 335916 Zebrafish
  • Omim: 165160 Human
  • SwissProt: P05412 Human
  • SwissProt: P05627 Mouse
  • SwissProt: P17325 Rat

  • SwissProt: Q4ZJE9 Zebrafish
  • SwissProt: Q6NZT5 Zebrafish
  • Unigene: 525704 Human
  • Unigene: 696684 Human
  • Unigene: 275071 Mouse
  • Unigene: 93714 Rat

  see all

• Alternative names
  Activator Protein 1 antibodyAP 1 antibodyAP1 antibody

  cJun antibodyEnhancer Binding Protein AP1 antibodyJun Activation Domain Binding Protein antibodyJUN antibodyJun oncogene antibodyJUN protein antibodyJun proto oncogene antibodyJUN_HUMAN antibodyJUNC antibodyOncogene JUN antibodyp39 antibodyProto oncogene c jun antibodyProto oncogene cJun antibodyProto-oncogene c-jun antibodyTranscription Factor AP 1 antibodyTranscription factor AP-1 antibodyTranscription Factor AP1 antibodyV jun avian sarcoma virus 17 oncogene homolog antibodyV jun sarcoma virus 17 oncogene homolog (avian) antibodyV jun sarcoma virus 17 oncogene homolog antibodyV-jun avian sarcoma virus 17 oncogene homolog antibodyvJun Avian Sarcoma Virus 17 Oncogene Homolog antibody

  see all

Anti-c-Jun (phospho S73) antibody images

• 

  Western blot - c-Jun (phospho S73) antibody (ab30620)
  All lanes : Anti-c-Jun (phospho S73) antibody (ab30620) at 1/500 dilution
  
  Lane 1 : extracts from untreated Hela cells
  Lane 2 : extracts from UV treated Hela cells
  
  
  Predicted band size : 36 kDa
  Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - c-Jun (phospho S73) antibody (ab30620)
  Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 Phospho c-Jun(Ser73) Antibody (ab30620). Left: untreated sample. Right: sample preincubated with synthesized phosphopeptide.
• 

  IHC - Wholemount - Anti-c-Jun (phospho S73) antibody (ab30620)This image is courtesy of an anonymous Abreview

  ab30620 staining 24hpf WT zebrafish embryos by IHC-wholemount. The embryos were dechorionated and fixed with 4% paraformaldehyde (or Dent's fixative) over 48h at 4 °C. Embryos were then washed two times with PBS and two times with MeOH, permeabilized with methanol for 5 minutes and blocked with 10% goat serum in PBS - Triton for 1 hour. Staining with ab30620 at a 1/100 dilution in PBS-10% goat serum- Triton was performed for 22h at 4°C. A goat anti-rabbit Alexa 568 polyclonal antibody at 1/1000 was used as the secondary antibody.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-c-Jun (phospho S73) antibody (ab30620)
  ICC/IF image of ab30620 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.