 image](#c-Jun-Primary-antibodies-ab13671-1.jpg)
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Product name
Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4]
See all c-Jun products (38) ...
Description
Mouse monoclonal [C-J 4C4] to c-Jun (phospho T91 + T93)
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
c-Jun protein phosphorylated at T91 and T93.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
C-J 4C4
Isotype
IgG1
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
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Immunocytochemistry/ Immunofluorescence-c-Jun (phospho T91 + T93) antibody [C-J 4C4](ab13671)
 image](/ps/datasheet/images/13/ab13671/c-Jun-Primary-antibodies-ab13671-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab13671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
- 1 Image
ICC/IF
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at an assay dependent dilution. Predicted molecular weight: 36 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Sequence similarities
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain.
Post-translational
modifications
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP05412
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activator Protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
Database links
- Entrez Gene: 3725 Human
- Entrez Gene: 24516 Rat
- Omim: 165160 Human
- SwissProt: P05412 Human
- SwissProt: P17325 Rat
- Unigene: 525704 Human
- Unigene: 696684 Human
- Unigene: 225373 Rat
see all
Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4] images:
Immunocytochemistry/ Immunofluorescence-c-Jun (phospho T91 + T93) antibody [C-J 4C4](ab13671)
ICC/IF image of ab13617 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13617, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-c-Jun (phospho T91 + T93) antibody [C-J 4C4] (ab13671)
This product has been referenced in:
- Madeo A et al. c-Jun activation is required for 4-hydroxytamoxifen-induced cell death in breast cancer cells. Oncogene 29:978-91 (2010). WB; Human.Read more (PubMed: 19935718) »
- Vinciguerra M et al. Negative charged threonine 95 of c-Jun is essential for c-Jun N-terminal kinase-dependent phosphorylation of threonine 91/93 and stress-induced c-Jun biological activity. Int J Biochem Cell Biol 40:307-16 (2008).Read more (PubMed: 17920329) »
See all 4 publications for this product
Publishing research using ab13671? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"