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Overview

  • Product namec-Kit Human ELISA KitSee all c-Kit kits ...
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 8 5.629ng/ml 0.34 6%
    Sample B 8 1.231ng/ml 0.06 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 8 5.44ng/ml 0.43 7.9%
    Sample B 8 1.314ng/ml 0.1 7.6%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 0.095 ng/ml
  • Range
    0.312 ng/ml - 10 ng/ml
  • Assay time
    1h 45m
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human c-Kit ELISA is to be used for the in-vitro quantitative determination of Stem Cell Factor receptor molecule (c-Kit) in human serum, plasma, buffered solutions or cell culture medium. This kit has been configured for research use only and is not to be used in diagnostic procedures.

    Principal:

    The c-Kit Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for c-Kit has been coated onto the wells of the microtiter strips provided. Samples, including standards of known c-Kit concentrations, specimens and unknowns are pipetted into these wells. During the first incubation, the c-Kit antigen and a biotinylated monoclonal antibody specific for c-Kit are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of c-Kit present in the samples.

     

    Recognizes both natural and recombinant human c-kit

    Range: 0.312 ng/ml - 10 ng/ml

    Sensitivity <0.095 ng/ml

  • Notes

    Safety Information:

    • For research use only.

    • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.

    • Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly with water.

    • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.

    • Do not pipette by mouth.

    • The stop solution in this kit contains 9.8% sulphuric acid.

  • Tested applicationsELISA more details

Properties

  • RelevanceKIT encodes the human homolog of the proto-oncogene c-kit. c-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.
  • Cellular localizationCell Membrane; single-pass type I membrane protein
    • Alternative names
        CD117 CD117 antigenEC 2.7.10.1
        Mast/stem cell growth factor receptor precursorPBT Proto oncogene tyrosine protein kinase KitSCFR v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab45924 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      c-Kit Human ELISA Kit images

      • ELISA - Human c-Kit ELISA Kit (ab45924)
        ELISA - Human c-Kit ELISA Kit (ab45924)
        Typical c-Kit standard curve ranging from 0.312 to 10 ng/ml.

      References for c-Kit Human ELISA Kit (ab45924)

      ab45924 has not yet been referenced specifically in any publications.

      Publishing research using ab45924 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab45924 Submit a Question for ab45924

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      Daer abcam technical support team

      i want to know about

      c-Kit Human ELISA Kit (ab45924)
      Ferritin Human ELISA Kit (ab108698)

      abcam sent the kit by format 96 well plate or strip in 96 well plate.
      please advise me.


      The c-Kit Human ELISA Kit (ab45924) and Ferritin Human ELISA Kit (ab108698) both come with strip wells provided.

      My customer would like to use Cerebrospinal Fluid (CSF) sample with c-Kit Human ELISA Kit (ab45924).

      May I check with u about the compatibility of ab45925 with CSF sample?

      Thank you for your enquiry.

      ab45925 is suitable for Cerebrospinal fluid.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"