c-Kit Human ELISA Kit (ab46505)

Overview

• Product namec-Kit Human ELISA KitSee all c-Kit kits ...
• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  A	8	5.629ng/ml	0.34	6%
  B	8	1.231ng/ml	0.06	4.8%

  Inter-assay

  Sample	n	Mean	SD	CV%
  A	8	5.44ng/ml	0.43	7.9%
  B	8	1.314ng/ml	0.1	7.6%

• Tests
  2 x 96 well plate
• Sample type
  Cell culture supernatant, Serum, Plasma
• Assay typeSandwich
• Sensitivity
  < 0.095 ng/ml
• Range
  0.312 ng/ml - 10 ng/ml
• Assay time
  1h 45m
• Species reactivity
  Reacts with: Human
  
• Product overview

  The Human c-Kit ELISA is to be used for the in-vitro quantitative determination of Stem Cell Factor receptor molecule (c-Kit) in human serum, plasma, buffered solutions or cell culture medium. This kit has been configured for research use only and is not to be used in diagnostic procedures.

  Principal:

  The c-Kit Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for c-Kit has been coated onto the wells of the microtiter strips provided. Samples, including standards of known c-Kit concentrations, specimens and unknowns are pipetted into these wells. During the first incubation, the c-Kit antigen and a biotinylated monoclonal antibody specific for c-Kit are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of c-Kit present in the samples.

   

  Recognizes both natural and recombinant human c-kit
  
  Range: 0.312 ng/ml - 10 ng/ml
  
  Sensitivity <0.095 ng/ml

• Notes

  Safety Notes:

  • For research use only.
  • The human blood components included in this kit have been tested and found non reactive for HBsAg and anti-HIV. Nevertheless, no known method can offer complete assurance that human blood derivatives will not transmit hepatitis, AIDS or other infections. Therefore, handling of reagents, serum or plasma specimens should be in accordance with local safety procedures , e.g. CDC/NIH Health manual : " Biosafety in Microbiological and Biomedical Laboratories" 1984.
  • Avoid any skin contact with H₂SO₄ and TMB. In case of contact, wash thoroughly with water.
  • Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
  • Do not pipette by mouth.
  • The stop solution in this kit contains 9.8% sulphuric acid.

• Tested applicationsELISA more details

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	Identifier	2 x 96 tests
  12 strips		8 units
  Biotinylated Antibody Diluent	Red	1 x 13ml
  Chromogen TMB		1 x 24ml
  Control	Silver	4 vials
  H2SO4: Stop Reagent	Black	2 x 11ml
  HRP Diluent	Red	1 x 23ml
  Standard	Yellow	4 vials
  Standard Diluent: Buffer.x10 Concentrated	Black	1 x 25ml
  Streptavidin-HRP		4 x 5µl
  Washing Buffer.x200 Concentrated	White	2 x 10ml

• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Growth factors

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Oncoprotein ELISA kits

  • Tags & Cell Markers
  • Cell Type Markers
  • Tumor Associated

Applications

c-Kit Human ELISA Kit images

• 

  ELISA - Human c-Kit ELISA Kit (ab46505)
  Typical c-Kit standard curve ranging from 0.312 to 10 ng/mL.

Protocols

• Human c-Kit ELISA Kit