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Anti-c-Kit antibody [2B8] (Allophycocyanin/Cy5.5 ® ) (ab46790)

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Overview

  • Product nameAnti-c-Kit antibody [2B8] (Allophycocyanin/Cy5.5 ® )See all c-Kit primary antibodies ...
  • Description
    Rat monoclonal [2B8] to c-Kit (Allophycocyanin/Cy5.5 ® )
  • ConjugationAllophycocyanin/Cy5.5 ®
  • Specificityab46790 recognizes CD117/c-Kit.
  • Tested applicationsFlow Cyt more details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Applications

Our Abpromise guarantee covers the use of ab46790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt
  • Application notesFlow Cyt: Use <0.2µg for 106 cells.
    Cell homing studies: Optimal dilutions/concentrations should be determined by the end user
    Hematopoiesis and development studies: Optimal dilutions/concentrations should be determined by the end user

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionTyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
    • Tissue specificityIsoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
    • Involvement in diseaseDefects in KIT are a cause of piebald trait (PBT) [MIM:172800]; also known as piebaldism. PBT is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes.
      Defects in KIT are a cause of gastrointestinal stromal tumor (GIST) [MIM:606764].
      Defects in KIT have been associated with testicular germ cell tumor (TGCT) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms.
      Defects in KIT are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients. These mutations fall into two classes, the most common being in-frame internal tandem duplications of variable length in the juxtamembrane region that disrupt the normal regulation of the kinase activity. Likewise, point mutations in the kinase domain can result in a constitutively activated kinase.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications      Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
      Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.
    • Cellular localizationCell membrane and Cytoplasm. Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.

      • Target information above from: UniProt accession P10721 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          C Kit antibodyc-Kit antibodyCD 117 antibody
          CD117 antibodyCD117 antigen antibodyKIT antibodyKIT oncogene antibodyKIT_HUMAN antibodyMast cell growth factor receptor antibodyMast/stem cell growth factor receptor antibodyMast/stem cell growth factor receptor Kit antibodyp145 c-kit antibodyPBT antibodyPiebald trait protein antibodyProto oncogene c Kit antibodyProto oncogene tyrosine protein kinase Kit antibodyProto-oncogene c-Kit antibodySCF Receptor antibodySCFR antibodysoluble KIT variant 1 antibodySteel Factor Receptor antibodyStem cell factor receptor antibodytyrosine protein kinase Kit antibodyTyrosine-protein kinase Kit antibodyv kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibodyv kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibodyv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody
        see all

      Anti-c-Kit antibody [2B8] (Allophycocyanin/Cy5.5 ® ) images

      • Flow Cytometry - c-Kit antibody [2B8] (Allophycocyanin/Cy5.5 ® ) (ab46790)
        Flow Cytometry - c-Kit antibody [2B8] (Allophycocyanin/Cy5.5 ® ) (ab46790)
        FACS analysis of BALB/c bone marrow cells double-stained with rat anti-mouse CD117-R-PE (ab46790) at 0.03 µg/106 cells and rat anti-mouse Ly-6G-FITC.

      References for Anti-c-Kit antibody [2B8] (Allophycocyanin/Cy5.5 ® ) (ab46790)

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