Anti-c-Kit (phospho Y703) antibody (ab62154)
Key features and details
- Rabbit polyclonal to c-Kit (phospho Y703)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-c-Kit (phospho Y703) antibody
See all c-Kit primary antibodies -
Description
Rabbit polyclonal to c-Kit (phospho Y703) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human c-Kit (phospho Y703). (A-L-YP-K-N)
Database link: P10721 -
Positive control
- WB: HepG2 treated with EGF cell lysate; Colon cancer lysate; U2OS lysate; Renal cancer cell line lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab62154 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 109 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 109 kDa. |
Target
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Function
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. -
Tissue specificity
Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells. -
Involvement in disease
Piebald trait
Gastrointestinal stromal tumor
Testicular germ cell tumor
Leukemia, acute myelogenous -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsUbiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7. -
Cellular localization
Cell membrane and Cytoplasm. Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head. - Information by UniProt
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Database links
- Entrez Gene: 3815 Human
- Entrez Gene: 16590 Mouse
- Omim: 164920 Human
- SwissProt: P10721 Human
- SwissProt: P05532 Mouse
- Unigene: 479754 Human
- Unigene: 247073 Mouse
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Alternative names
- C Kit antibody
- c-Kit antibody
- c-Kit Ligand antibody
see all
Images
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All lanes : Anti-c-Kit (phospho Y703) antibody (ab62154) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) treated with EGF (200ng/ml, 30mins) cell lysate
Lane 2 : HepG2 treated with EGF (200ng/ml, 30mins) cell lysate with immunizing phosphopeptide
Predicted band size: 109 kDa -
All lanes : Anti-c-Kit (phospho Y703) antibody (ab62154)
Lane 1 : Colon cancer lysate (human)
Lane 2 : U2OS lysate (human)
Lane 3 : Renal cancer cell line (human)
Secondary
All lanes : Swine anti-rabbit HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 109 kDa
Exposure time: 6 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab62154 has been referenced in 8 publications.
- Shima T et al. Glucose transporter‑1 inhibition overcomes imatinib resistance in gastrointestinal stromal tumor cells. Oncol Rep 47:N/A (2022). PubMed: 34738628
- Zhao X et al. Lactobacillus plantarum YS2 (yak yogurt Lactobacillus) exhibited an activity to attenuate activated carbon-induced constipation in male Kunming mice. J Dairy Sci 102:26-36 (2019). PubMed: 30527985
- Yang H et al. Predictive and prognostic value of phosphorylated c-KIT and PDGFRA in advanced non-small cell lung cancer harboring ALK fusion. Oncol Lett 17:3071-3076 (2019). PubMed: 30867736
- Gong Y et al. SCF/SCFR signaling plays an important role in the early morphogenesis and neurogenesis of human embryonic neural retina. Development 146:N/A (2019). PubMed: 31548215
- Li G et al. Preventative effects of fermented Chimonobambusa quadrangularis shoot on activated carbon-induced constipation. Exp Ther Med 13:1093-1100 (2017). WB . PubMed: 28450948
- Yang S et al. BMP4 promotes mouse iPS cell differentiation to male germ cells via Smad1/5, Gata4, Id1 and Id2. Reproduction 153:211-220 (2017). PubMed: 27864336
- Grewal N et al. Histopathology of aortic complications in bicuspid aortic valve versus Marfan syndrome: relevance for therapy? Heart Vessels 31:795-806 (2016). PubMed: 26129868
- Grewal N et al. Bicuspid aortic valve: phosphorylation of c-Kit and downstream targets are prognostic for future aortopathy. Eur J Cardiothorac Surg 46:831-9 (2014). PubMed: 25161185