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Anti-c-Maf antibody (ab76817) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
Predicted band size : 42 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
MW of GST tag alone: 26 kDa
Anti-c-Maf antibody (ab76817) at 5 µg/ml + PC-12 cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size : 42 kDa
Observed band size : 42 kDa
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