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Western blot - c-Maf antibody (ab76817)
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Western blot - c-Maf antibody (ab76817)
(enlarge)
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Product Name
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c-Maf antibody
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See all c-Maf antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to c-Maf
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Immunogen
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GST-tagged recombinant fragment: SCRFKRVQQR HVLESEKNQL LQQVDHLKQE ISRLVRERDA YKEKYEKLVS SGFRENGSSS DNPSSPEFFI TEPTRKLEPS VGYATFWKPQ HRVLTSVFTK , corresponding to amino acids 304-404 of Human c-Maf
BLAST 'SCRFKRVQQR HVLESEKNQL LQQVDHLKQE ISRLVRERDA YKEKYEKLVS SGFRENGSSS DNPSSPEFFI TEPTRKLEPS VGYATFWKPQ HRVLTSVFTK ' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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PC-12 cell lysate
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Immunology >> Immune System Diseases >> Allergens
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Leucine Zipper
Cell Biology >> Apoptosis >> Extracellular Signals >> Death Ligands
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Relevance
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Members of the small Maf family are basic region leucine zipper (bZIP) proteins that can function as transcriptional activators or repressors. Small Maf proteins can switch from transcriptional repressors to activators, depending on the proteins with which they form heterodimers.
MAF chromosomal abnormalities are found in some types of multiple melanomas.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2b
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Light chain type
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kappa
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.2
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.50 - 1.00 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this c-Maf antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for c-Maf antibody images, references, products related to ab76817 and other tools.
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c-Maf antibody images:
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 Western blot - c-Maf antibody (ab76817)
c-Maf antibody (ab76817) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
Predicted band size : 42 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
MW of GST tag alone: 26 kDa
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Western blot - c-Maf antibody (ab76817)
c-Maf antibody (ab76817) at 5 µg/ml + PC-12 cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size : 42 kDa
Observed band size : 42 kDa
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Search PubMed (MEDLINE) for references to c-Maf
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c-Maf antibody - more information
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Customer reviews (feedback) regarding c-Maf antibody |
Customer FAQs regarding c-Maf antibody |
Protocols for c-Maf antibody |
Price and availability of products related to c-Maf antibody |
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c-Maf antibody - related products:
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c-Maf antibody - other tools:
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Contact Abcam with a Technical Enquiry about c-Maf antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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