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Synthetic peptide corresponding to Human c-Myc aa 400-500 (C terminal) conjugated to keyhole limpet haemocyanin.
Our Abpromise guarantee covers the use of ab32 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|ChIP||Use at an assay dependent concentration.|
|IHC (Methanol fixed)||1/200. PubMed: 17329357|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/1000.|
|IP||Use at 6 µg/mg of lysate.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||1/250 - 1/500.|
|IHC-Fr||1/1000. See Abreviews.|
|Purification||Use at an assay dependent concentration.|
ab32 staining c-Myc in human esophagus tissue sections by immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a citric buffer. Samples were incubated with primary antibody (1/300) for 12 hours at 4°C. A HRP-conjugated rabbit anti-mouse polyclonal (1/200) was used as the secondary antibody.
Menssen R et al., (2001) Curr Biol. Mar 6;11(5):345-50.
Phosphorylation of Cdc15 changes during the cell cycle. Exponentially growing cells (cyc) of CDC15-MYC9 (W1114) were arrested in G1 with a factor pheromone and released into fresh medium at 25°C. Cells were harvested at the indicated times, the percentage of divided nuclei was determined by DAPI staining of fixed cells, and proteins were analyzed by western blotting with 9E10 (ab32).
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