• Product nameAnti-c-Myc antibody [9E10] (HRP)
    See all c-Myc primary antibodies
  • Description
    Mouse monoclonal [9E10] to c-Myc (HRP)
  • ConjugationHRP
  • Tested applicationsSuitable for: ELISA, IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human c-Myc aa 400 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control



Our Abpromise guarantee covers the use of ab62928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/100 - 1/500.
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
WB 1/10000 - 1/50000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).


  • FunctionParticipates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • Involvement in diseaseNote=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Formc-Myc is also expressed in the cytoplasm.
  • Alternative names
    • AU016757 antibody
    • Avian myelocytomatosis viral oncogene homolog antibody
    • bHLHe39 antibody
    • c Myc antibody
    • Class E basic helix-loop-helix protein 39 antibody
    • MRTL antibody
    • Myc antibody
    • Myc protein antibody
    • Myc proto oncogene protein antibody
    • Myc proto-oncogene protein antibody
    • myc-related translation/localization regulatory factor antibody
    • MYC_HUMAN antibody
    • Myc2 antibody
    • MYCC antibody
    • Myelocytomatosis oncogene antibody
    • Niard antibody
    • Nird antibody
    • Oncogene Myc antibody
    • OTTHUMP00000158589 antibody
    • Proto-oncogene c-Myc antibody
    • Protooncogene homologous to myelocytomatosis virus antibody
    • RNCMYC antibody
    • Transcription factor p64 antibody
    • Transcriptional regulator Myc-A antibody
    • V-Myc avian myelocytomatosis viral oncogene homolog antibody
    • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

Anti-c-Myc antibody [9E10] (HRP) images

  • Anti-c-Myc antibody [9E10] (HRP) (ab62928) at 1/10000 dilution + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa

References for Anti-c-Myc antibody [9E10] (HRP) (ab62928)

This product has been referenced in:
  • Huang J  et al. Linc-RoR promotes c-Myc expression through hnRNP I and AUF1. Nucleic Acids Res 44:3059-69 (2016). RT-PCR . Read more (PubMed: 26656491) »
  • Vivante A  et al. Mutations in TBX18 Cause Dominant Urinary Tract Malformations via Transcriptional Dysregulation of Ureter Development. Am J Hum Genet 97:291-301 (2015). WB . Read more (PubMed: 26235987) »

See all 12 Publications for this product

Product Wall

The laboratory confirmed that they tested 0.1ug E. coli with myc-tag in WB and they obtained a good signal at 1 min exposure. However, they did not test the limits of ab62928 products sensitivity, but they would expect it to be able to detect smaller q...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Recombinant protein (BL21(DE3))
Loading amount 0.5 µg
Specification BL21(DE3)
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T cells)
Loading amount 20 µg
Specification 293T cells
Gel Running Conditions Reduced Denaturing (Invitrogen NUPAGE Bis-Tris Gel 4-12% Gradient Gel MES Buffer)
Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 25 2010