Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab62928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-P
IHC-Fr
WB
  • Application notesELISA: 1/100 - 1/500.
    IHC-P: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    WB: 1/10000 - 1/50000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

    Unconjugated version of this antibody available as ab32.
    FITC conjugated version of this antibody available as ab64478.
    Biotin conjugated version of this antibody available as ab10825.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionParticipates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
    • Involvement in diseaseNote=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
      Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
      Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
    • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      modifications
      Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
      Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
    • Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Formc-Myc is also expressed in the cytoplasm.
    • Alternative names
      • AU016757 antibody
      • Avian myelocytomatosis viral oncogene homolog antibody
      • bHLHe39 antibody
      • c Myc antibody
      • c Myc oncoprotein antibody
      • Cellular myelocytomatosis oncogene antibody
      • Class E basic helix-loop-helix protein 39 antibody
      • MRTL antibody
      • Myc antibody
      • Myc protein antibody
      • Myc proto oncogene protein antibody
      • Myc proto-oncogene protein antibody
      • myc-related translation/localization regulatory factor antibody
      • MYC_HUMAN antibody
      • Myc2 antibody
      • MYCC antibody
      • Myelocytomatosis oncogene antibody
      • Niard antibody
      • Nird antibody
      • Oncogene Myc antibody
      • OTTHUMP00000158589 antibody
      • Proto-oncogene c-Myc antibody
      • Protooncogene homologous to myelocytomatosis virus antibody
      • RNCMYC antibody
      • Transcription factor p64 antibody
      • Transcriptional regulator Myc-A antibody
      • v myc avian myelocytomatosis viral oncogene homolog antibody
      • v myc myelocytomatosis viral oncogene homolog (avian) antibody
      • V-Myc avian myelocytomatosis viral oncogene homolog antibody
      • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
      see all

    Anti-c-Myc antibody [9E10] (HRP) images

    • Anti-c-Myc antibody [9E10] (HRP) (ab62928) at 1/10000 dilution + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.1 µg

      Performed under reducing conditions.

      Predicted band size : 41 kDa
      Observed band size : 41 kDa

    References for Anti-c-Myc antibody [9E10] (HRP) (ab62928)

    This product has been referenced in:
    • Cunningham C  et al. Isolation and characterisation of a recombinant antibody fragment that binds NCAM1-expressing intervertebral disc cells. PLoS One 8:e83678 (2013). ELISA . Read more (PubMed: 24349544) »
    • Hauler F  et al. AAA ATPase p97/VCP is essential for TRIM21-mediated virus neutralization. Proc Natl Acad Sci U S A 109:19733-8 (2012). Read more (PubMed: 23091005) »

    See all 6 Publications for this product

    Product Wall

    The laboratory confirmed that they tested 0.1ug E. coli with myc-tag in WB and they obtained a good signal at 1 min exposure. However, they did not test the limits of ab62928 products sensitivity, but they would expect it to be able to detect smaller q...

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    Thank you for contacting us.

    As requested I am sending you the following links:

    Human Protein Atlas:

    http://www.proteinatlas.org/ENSG00000136997
    http://www.proteinatlas.org/ENSG00000136997/normal


    Datasheets ...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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    Thank you for your note. This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab62928. For your information, the internal reference note for this credit is ...

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    Thank you for getting back to me and for the updates. It is very likely that the antibody is inactive or due to some sterical hindrance it does not recognize the epitope. I have checked our current stock and unfortunately we do not have new batch...

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    Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. As the datasheet indicates ab62928 was raised against...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Escherichia coli Recombinant protein (BL21(DE3))
    Loading amount 0.5 µg
    Specification BL21(DE3)
    Gel Running Conditions Reduced Denaturing (4-20%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 30 2010

    Application Western blot
    Sample Human Cell lysate - whole cell (293T cells)
    Loading amount 20 µg
    Specification 293T cells
    Gel Running Conditions Reduced Denaturing (Invitrogen NUPAGE Bis-Tris Gel 4-12% Gradient Gel MES Buffer)
    Blocking step Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"