Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
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ab46666 |
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ab56 has been referenced in 14 publications.
Publishing research using ab56? Please let us know so that we can cite the reference in this datasheet
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Overlay histogram showing HL60 cells stained with ab56 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
Anti-c-Myc antibody [9E11] - ChIP Grade (ab56) at 1/1000 dilution + whole cell lysate prepared from huh-7 cells at 15 µg
Secondary
Anti-mouse IgG conjugated to HRP at 1/10000 dilution
Predicted band size : 49 kDa
Image courtesy of an anonymous Abreview.
Anti-c-Myc antibody [9E11] - ChIP Grade (ab56) at 1/500 dilution +
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
3
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