Anti-Myc tag antibody [JAC6] (ab10910)
Key features and details
- Rat monoclonal [JAC6] to c-Myc
- Suitable for: IHC-Fr, IP, WB
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Myc tag antibody [JAC6]
See all c-Myc primary antibodies -
Description
Rat monoclonal [JAC6] to c-Myc -
Host species
Rat -
Specificity
This antibody recognises the myc epitope which is frequently used to tag proteins, and is the same epitope recognised by clone 9E10. -
Tested applications
Suitable for: IHC-Fr, IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human c-Myc aa 408-439.
Sequence:AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA
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General notes
This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatent. -
Clonality
Monoclonal -
Clone number
JAC6 -
Isotype
IgG1 -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. -
Involvement in disease
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Post-translational
modificationsPhosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. -
Cellular localization
Nucleus > nucleoplasm. Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 4609 Human
- Omim: 190080 Human
- SwissProt: P01106 Human
- Unigene: 202453 Human
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Form
c-Myc is also expressed in the cytoplasm. -
Alternative names
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (10)
ab10910 has been referenced in 10 publications.
- Tsai PI et al. PINK1 Phosphorylates MIC60/Mitofilin to Control Structural Plasticity of Mitochondrial Crista Junctions. Mol Cell 69:744-756.e6 (2018). PubMed: 29456190
- Garner SRC et al. Drosophila female-specific Ilp7 motoneurons are generated by Fruitless-dependent cell death in males and by a double-assurance survival role for Transformer in females. Development 145:N/A (2018). PubMed: 29229771
- Bookwalter CS et al. Reconstitution of the core of the malaria parasite glideosome with recombinant Plasmodium class XIV myosin A and Plasmodium actin. J Biol Chem 292:19290-19303 (2017). IF . PubMed: 28978649
- Su X et al. Regulation of polycystin-1 ciliary trafficking by motifs at its C-terminus and polycystin-2 but not by cleavage at the GPS site. J Cell Sci 128:4063-73 (2015). IF ; Mouse . PubMed: 26430213
- von Philipsborn AC et al. Cellular and behavioral functions of fruitless isoforms in Drosophila courtship. Curr Biol 24:242-51 (2014). IHC . PubMed: 24440391
- Coleman JA & Molday RS Critical Role of the {beta}-Subunit CDC50A in the Stable Expression, Assembly, Subcellular Localization, and Lipid Transport Activity of the P4-ATPase ATP8A2. J Biol Chem 286:17205-16 (2011). IHC-Fr . PubMed: 21454556
- Ringli C The hydroxyproline-rich glycoprotein domain of the Arabidopsis LRX1 requires Tyr for function but not for insolubilization in the cell wall. Plant J : (2010). WB . PubMed: 20545889
- Bushell ES et al. Paternal effect of the nuclear formin-like protein MISFIT on Plasmodium development in the mosquito vector. PLoS Pathog 5:e1000539 (2009). WB . PubMed: 19662167
- Fernando NT et al. Tumor escape from endogenous, extracellular matrix-associated angiogenesis inhibitors by up-regulation of multiple proangiogenic factors. Clin Cancer Res 14:1529-39 (2008). IHC-Fr . PubMed: 18316578
- Zavortink M et al. Tum/RacGAP50C provides a critical link between anaphase microtubules and the assembly of the contractile ring in Drosophila melanogaster. J Cell Sci 118:5381-92 (2005). PubMed: 16280552