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ab32072 has been referenced in 6 publications.
Publishing research using ab32072? Please let us know so that we can cite the reference in this datasheet
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ab32072 staining c-Myc in HEK-293 cells transfected with CACNB4-c-Myc by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100 then blocked using 5% serum for 20 minutes at 25°C. Samples were then incubated with ab32072 at a 1/250 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.
Image courtesy of Dr Vladimir Milenkovic by Abreview.
Anti-c-Myc antibody [Y69] (ab32072) at 1/10000 dilution + Jurkat cell lysate.
Predicted band size : 49 kDa
Observed band size : 57 kDa (why is the actual band size different from the predicted?)
Ab32072, at a dilution of 1/250, staining Hela cells by Immunoflourescence.
Ab32072, at a dilution of 1/50, staining c-Myc in paraffin embedded human skin carcinoma tissue by Immunohistochemistry.
ICC/IF image of ab32072 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32072, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab32072 showing positive staining in Colonic adenocarcinoma tissue.
ab32072 showing positive staining in Urinary bladder transitional carcinoma tissue.
ab32072 showing positive staining in Lung adenocarcinoma tissue.
ab32072 showing positive staining in Gastric adenocarcinoma tissue.
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