Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
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Thank you for your assistance. |
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ANSWER: |
Thank you for kindly confirming these details. I am sorry these antibodies have not worked for this customer. |
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Inquiry: Hello, Please could you tell me the exact amino acid sequence of the epitope used to generate the following antibody: Anti-c-Myc antibody [Y69] (ab32072) Many thanks! |
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ANSWER: |
Thank you for your enquiry and your interest. |
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Dear Technical Team, |
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ANSWER: |
Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear the customer hashad difficulty obtaining satisfactory results from the free of charge replacement vials. |
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Could you send me your MOM protocol, and the datasheets for ab32072 and ab6285. |
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ANSWER: |
Thank you for your inquiry and I apologize that we were not able to receive your earlier phone call. |
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Thanks. It is great if you can send me a hard copy of these inserts.. Regards Shally : 10/27/11 |
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ANSWER: |
Thank you for your reply. I will be happy to mail the datasheets to you. Are you having trouble accessing the datasheets online? I only ask so that I can inform our IT department if there are problems. Could you also please send me the address that you would like the datasheets sent to. Thank you and I look forward to your reply. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab32072 staining c-Myc in HEK-293 cells transfected with CACNB4-c-Myc by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100 then blocked using 5% serum for 20 minutes at 25°C. Samples were then incubated with ab32072 at a 1/250 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.
Image courtesy of Dr Vladimir Milenkovic by Abreview.
Anti-c-Myc antibody [Y69] (ab32072) at 1/10000 dilution + Jurkat cell lysate.
Predicted band size : 49 kDa
Observed band size : 57 kDa (why is the actual band size different from the predicted?)
Ab32072, at a dilution of 1/250, staining Hela cells by Immunoflourescence.
Ab32072, at a dilution of 1/50, staining c-Myc in paraffin embedded human skin carcinoma tissue by Immunohistochemistry.
ICC/IF image of ab32072 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32072, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab32072 showing positive staining in Colonic adenocarcinoma tissue.
ab32072 showing positive staining in Urinary bladder transitional carcinoma tissue.
ab32072 showing positive staining in Lung adenocarcinoma tissue.
ab32072 showing positive staining in Gastric adenocarcinoma tissue.
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