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Western blot - c-Myc antibody [Y69] (ab32072)
![Western blot - c-Myc antibody [Y69] (ab32072)](/ps/datasheet/images/ab32072_1.jpg)
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Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69] (ab32072)
![Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69] (ab32072)](/ps/datasheet/images/ab32072_2.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - c-Myc antibody [Y69] (ab32072)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - c-Myc antibody [Y69] (ab32072)](/ps/datasheet/images/ab32072_3.jpg)
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Product Name
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c-Myc antibody [Y69]
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See all c-Myc antibodies (37)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [Y69] to c-Myc
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Immunogen
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Synthetic peptide corresponding to residues in the N terminus of Human c-Myc.
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Rat
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Specificity
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This antibody is specific for c-Myc.
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Tested applications
(see key)
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ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ICC/IF: 1/250 - 1/500. IHC-P: 1/50. IP: 1/150. WB: 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 49 kDa).
Is unsuitable for FACS.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: Jurkat cell lysate. IF: Hela cells. IHC-P: Human skin carcinoma.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Cancer >> Tumor biomarkers >> Oncoproteins Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors Cancer >> Cell cycle >> Cell differentiation Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
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Relevance
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The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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Y69
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Isotype
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IgG
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Storage buffer
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Preservative: 0.01% Sodium Azide. Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4. Material safety datasheets (MSDS) for this product: Glycerol MSDS Sodium Azide MSDS
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Form
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Liquid
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this c-Myc antibody [Y69] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for c-Myc antibody [Y69] images, references, products related to ab32072 and other tools.
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c-Myc antibody [Y69] images:
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Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69] (ab32072)
Ab32072, at a dilution of 1/250, staining Hela cells by Immunoflourescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - c-Myc antibody [Y69] (ab32072)
Ab32072, at a dilution of 1/50, staining c-Myc in paraffin embedded human skin carcinoma tissue by Immunohistochemistry.
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References for c-Myc antibody [Y69] (ab32072)
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This product has been used in: (two most recent references)
Escamilla-Powers JR et al. The tumor suppressor protein HBP1 is a novel c-myc-binding protein that negatively regulates c-myc transcriptional activity. J Biol Chem 285:4847-58 (2010). IP; Human. PubMed: 20008325
Rohan JN & Weigel NL 1,25(OH)2D3 reduces c-Myc expression, inhibiting proliferation and causing G1 accumulation in C4-2 prostate cancer cells. Endocrinology : (2009). PubMed: 19164469
If you publish research using ab32072 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to c-Myc
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c-Myc antibody [Y69] - more information
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Customer reviews (feedback) regarding c-Myc antibody [Y69] |
Customer FAQs regarding c-Myc antibody [Y69] |
Protocols for c-Myc antibody [Y69] |
Price and availability of products related to c-Myc antibody [Y69] |
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c-Myc antibody [Y69] - related products:
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c-Myc antibody [Y69] - other tools:
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Contact Abcam with a Technical Enquiry about c-Myc antibody [Y69] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"