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Anti-c-Myc antibody [Y69] (ab32072)

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Overview

Product name

Anti-c-Myc antibody [Y69]
See all c-Myc products (42) ...

Description

Rabbit monoclonal [Y69] to c-Myc

Specificity

This antibody is specific for c-Myc.

Tested applications

WB, IHC-P, ICC/IF, IHC-P, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to residues in the N terminus of Human c-Myc.

Positive control

WB: Jurkat cell lysate. IF: Hela cells. IHC-P: Human skin carcinoma.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide.
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4.

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

Y69

Isotype

IgG

  • Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69] (ab32072)Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69] (ab32072) image (enlarge)

  • Western blot - c-Myc antibody [Y69] (ab32072)Western blot - c-Myc antibody [Y69] (ab32072) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69] (ab32072)Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69] (ab32072) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/250 - 1/500.
IHC-P: 1/50.
IP: 1/150.
WB: 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 49 kDa).

Is unsuitable for FACS.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.

Involvement in disease

Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.

Sequence similarities

Contains 1 basic helix-loop-helix (bHLH) domain.

Post-translational
modifications

Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.

Cellular localization

Nucleus > nucleoplasm. Nucleus > nucleolus.

Target information above from: UniProt accessionP01106 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AU016757 antibody
  • Avian myelocytomatosis viral oncogene homolog antibody
  • bHLHe39 antibody
  • c Myc antibody
  • Cellular myelocytomatosis oncogene antibody
  • Cellular myelocytomatosis oncogene antibody
  • Class E basic helix-loop-helix protein 39 antibody
  • MGC105490 antibody
  • MRTL antibody
  • Myc antibody
  • Myc protein antibody
  • Myc proto oncogene protein antibody
  • Myc proto oncogene protein antibody
  • Myc proto-oncogene protein antibody
  • Myc-related translation/localization regulatory factor antibody
  • MYC_HUMAN antibody
  • Myc2 antibody
  • myca antibody
  • Myelocytomatosis oncogene a antibody
  • Myelocytomatosis oncogene antibody
  • Niard antibody
  • Nird antibody
  • Oncogene Myc antibody
  • OTTHUMP00000158589 antibody
  • OTTHUMP00000227763 antibody
  • Proto-oncogene c-Myc antibody
  • Protooncogene homologous to myelocytomatosis virus antibody
  • RNCMYC antibody
  • Transcription factor p64 antibody
  • Transcriptional regulator Myc-A antibody
  • v myc avian myelocytomatosis viral oncogene homolog antibody
  • v myc myelocytomatosis viral oncogene homolog (avian) antibody
  • V-Myc avian myelocytomatosis viral oncogene homolog antibody
  • v-myc myelocytomatosis viral oncogene homolog (avian) antibody
  • zc-myc antibody
see all

Anti-c-Myc antibody [Y69] images:

  Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69] (ab32072)

Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69] (ab32072)

ab32072 staining c-Myc in HEK-293 cells transfected with CACNB4-c-Myc by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100 then blocked using 5% serum for 20 minutes at 25°C. Samples were then incubated with ab32072 at a 1/250 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

Image courtesy of Dr Vladimir Milenkovic by Abreview.

See Abreview

  Western blot - c-Myc antibody [Y69] (ab32072)

Western blot - c-Myc antibody [Y69] (ab32072)

Anti-c-Myc antibody [Y69] (ab32072) at 1/10000 dilution + Jurkat cell lysate.

Predicted band size : 49 kDa
Observed band size : 57 kDa (why is the actual band size different from the predicted?)

  Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69] (ab32072)

Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69] (ab32072)

Ab32072, at a dilution of 1/250, staining Hela cells by Immunoflourescence.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - c-Myc antibody [Y69] (ab32072)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - c-Myc antibody [Y69] (ab32072)

Ab32072, at a dilution of 1/50, staining c-Myc in paraffin embedded human skin carcinoma tissue by Immunohistochemistry.

  Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69] (ab32072)

Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69] (ab32072)

ICC/IF image of ab32072 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32072, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-c-Myc antibody [Y69] (ab32072)

This product has been referenced in:

  • Scholer-Dahirel Aet al. Maintenance of adenomatous polyposis coli (APC)-mutant colorectal cancer is dependent on Wnt/{beta}-catenin signaling. Proc Natl Acad Sci U S A 108:17135-40 (2011). IHC-P; Human.Read more (PubMed: 21949247) »
  • Bruning Uet al. MicroRNA-155 promotes resolution of hypoxia-inducible factor 1alpha activity during prolonged hypoxia. Mol Cell Biol 31:4087-96 (2011).Read more (PubMed: 21807897) »

See all 4 publications for this product

Publishing research using ab32072? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"