c-Myc antibody [Y69] (ab32072)

Product Name 

c-Myc antibody [Y69]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [Y69] to c-Myc

Immunogen 

Synthetic peptide corresponding to residues in the N terminus of Human c-Myc.

Reacts with 

Hu

Does not react with 
(species key)

Ms, Rat

Specificity 

This antibody is specific for c-Myc.

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/250 - 1/500.
IHC-P: 1/50.
IP: 1/150.
WB: 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 49 kDa).

Is unsuitable for FACS.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: Jurkat cell lysate.
IF: Hela cells.
IHC-P: Human skin carcinoma.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Cancer >> Cell cycle >> Cell differentiation
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper

Relevance 

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

Y69

Isotype 

IgG

Storage buffer 

Preservative: 0.01% Sodium Azide.
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4.

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

See below for c-Myc antibody [Y69] images, references, products related to ab32072 and other tools.

c-Myc antibody [Y69] images:

References for c-Myc antibody [Y69] (ab32072)

  This product has been used in: (two most recent references)

Escamilla-Powers JR et al. The tumor suppressor protein HBP1 is a novel c-myc-binding protein that negatively regulates c-myc transcriptional activity. J Biol Chem 285:4847-58 (2010). IP; Human. PubMed: 20008325

Rohan JN & Weigel NL 1,25(OH)2D3 reduces c-Myc expression, inhibiting proliferation and causing G1 accumulation in C4-2 prostate cancer cells. Endocrinology : (2009). PubMed: 19164469
If you publish research using ab32072 please let us know so that we can cite the reference on this datasheet.

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