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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Overview
Product name
Anti-c-Myc antibody
See all c-Myc products (42) ...
Description
Rabbit polyclonal to c-Myc
Specificity
This antibody recognizes the 65kDa form of c-Myc.
Tested applications
Cross reactivity
Reacts with
Mouse, Human, Monkey
Predicted to work with
Sheep, Rabbit, Cat, Dog, Pig, Chimpanzee
Immunogen
Synthetic peptide: NRNYDLDYDSVQPY, corresponding to amino acids 9-22 of Human c-Myc.
NRNYDLDYDS VQPY
Positive control
3T6 Swiss albino nuclear extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide and 0.01% Thiomerosal.
Constituents: PBS containing 0.2% Gelatin.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Cancer >> Cell cycle >> Cell differentiation
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
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Western blot - Anti-c-Myc antibody (ab11917)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab11917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 65 kDa (predicted molecular weight: 49 kDa).
Target
Function
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Involvement in disease
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
Cellular localization
Nucleus > nucleoplasm. Nucleus > nucleolus.
Target information above from: UniProt accessionP01106
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
see all
Database links
- Entrez Gene: 464393 Chimpanzee
- Entrez Gene: 4609 Human
- Entrez Gene: 17869 Mouse
- Omim: 190080 Human
- SwissProt: P23583 Chimpanzee
- SwissProt: Q28350 Dog
- SwissProt: P01106 Human
- SwissProt: P01108 Mouse
see all
Anti-c-Myc antibody images:
Western blot - Anti-c-Myc antibody (ab11917)
Predicted band size : 49 kDa
Detection of c-Myc by Western blot analysis. A 65 kDa form of the transcription factor c-Myc is detected in nuclear extracts derived from 3T6 Swiss Albino cells using c-Myc rabbit polyclonal antibody at a 1/1000 dilution. The signal corresponding to nuclear c-Myc observed in lane 1 is eliminated when the immunizing peptide is added to the reaction mixture.
References for Anti-c-Myc antibody (ab11917)
This product has been referenced in:
- Arlt VMet al. Gene expression changes induced by the human carcinogen aristolochic acid I in renal and hepatic tissue of mice. Int J Cancer 128:21-32 (2011).Read more (PubMed: 20232387) »
- Yusa Ket al. Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon. Nat Methods 6:363-9 (2009). WB; Human.Read more (PubMed: 19337237) »
See all 5 publications for this product
Publishing research using ab11917? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"