c-Myc antibody (ab11917)

Product Name 

c-Myc antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to c-Myc

Immunogen 

Synthetic peptide: NRNYDLDYDSVQPY, corresponding to amino acids 9-22 of Human c-Myc.
BLAST 'NRNYDLDYDSVQPY' with or

Reacts with 

Hu, Ms, Mk

Predicted to react 
(species key)

Cat, Chmp, Dog, Pig, Rb, Shp
(Due to sequence homology)

Specificity 

This antibody recognizes the 65kDa form of c-Myc.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 49 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

3T6 Swiss albino nuclear extract.

Cellular localization 

Cytoplasmic and Nuclear

Research areas 

Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Cancer >> Cell cycle >> Cell differentiation
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper

Relevance 

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide and 0.01% Thiomerosal.
Constituents: PBS containing 0.2% Gelatin.

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for c-Myc antibody images, references, products related to ab11917 and other tools.

c-Myc antibody images:

References for c-Myc antibody (ab11917)

  This product has been used in: (two most recent references)

Yusa K et al. Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon. Nat Methods 6:363-9 (2009). WB; Human. PubMed: 19337237

Li X et al. Oncogenesis of T-ALL and nonmalignant consequences of overexpressing intracellular NOTCH1. J Exp Med 205:2851-61 (2008). PubMed: 18981238

See all 4 publication references for this product.


If you publish research using ab11917 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding c-Myc antibody

Customer FAQs regarding c-Myc antibody

Protocols for c-Myc antibody

Price and availability of products related to c-Myc antibody

c-Myc antibody - related products: