Anti-Myc tag antibody (ab19233)
Key features and details
- Chicken polyclonal to Myc tag
- Suitable for: WB
- Isotype: IgY
Overview
-
Product name
Anti-Myc tag antibody
See all Myc tag primary antibodies -
Description
Chicken polyclonal to Myc tag -
Host species
Chicken -
Tested applications
Suitable for: WBmore details -
Immunogen
Synthetic peptide corresponding to Human Myc tag aa 400-500 conjugated to keyhole limpet haemocyanin.
Database link: P01106 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
-
Assay kits
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 49 kDa. |
Target
-
Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
Cellular localization
Nuclear -
Alternative names
- c-myc tag antibody
- Myc Epitope Tag antibody
Images
-
Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, chicken anti-c-myc antibody (ab19233) used for WB at 0.2 µg/ml (1:5,000). Detection: Chemiluminescence with an exposure time of 10 seconds.
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab19233 has been referenced in 4 publications.
- Del Toro D et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell 180:323-339.e19 (2020). PubMed: 31928845
- Dagan B et al. A hyperthermophilic protein G variant engineered via directed evolution prevents the formation of toxic SOD1 oligomers. Proteins N/A:N/A (2019). PubMed: 31017342
- Jackson VA et al. Super-complexes of adhesion GPCRs and neural guidance receptors. Nat Commun 7:11184 (2016). WB ; Human . PubMed: 27091502
- Pastuszka MK et al. Flipping the Switch on Clathrin-Mediated Endocytosis using Thermally Responsive Protein Microdomains. Adv Funct Mater 24:5340-5347 (2014). PubMed: 25419208