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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Overview
Product name
Anti-c-Myc antibody
See all c-Myc products (42) ...
Description
Rabbit polyclonal to c-Myc
Specificity
This antibody detects endogenous levels of total Myc protein.
Tested applications
ICC/IF, IHC-P, WB, IHC-P, ELISA, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic non-phosphopeptide derived from Human Myc around the phosphorylation site of threonine 358.
Positive control
Human breast carcinoma tissue; NIH/3T3 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Oncoproteins
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Transcription factors
Cancer >> Cell cycle >> Cell differentiation
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Signaling Pathways >> TGF beta >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: 1/20,000.
IHC-P: 1/50 - 1/100.
IHC-FoFr: Use at an assay dependent dilution (PMID 18948956).
WB: 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Involvement in disease
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
Cellular localization
Nucleus > nucleoplasm. Nucleus > nucleolus.
Target information above from: UniProt accessionP01106
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
see all
Database links
- Entrez Gene: 4609 Human
- Entrez Gene: 17869 Mouse
- Entrez Gene: 24577 Rat
- Omim: 190080 Human
- SwissProt: P01106 Human
- SwissProt: P01108 Mouse
- SwissProt: P09416 Rat
- Unigene: 202453 Human
see all
Anti-c-Myc antibody images:
Western blot - c-Myc antibody (ab39688)
All lanes : Anti-c-Myc antibody (ab39688) at 1/500 dilution
Lane 1 : NIH/3T3 cell extracts
Lane 2 : NIH/3T3 cell extracts with synthesized peptide
Predicted band size : 49 kDa
Observed band size : 49 kDa
Western blot analysis of extracts from NIH/3T3 cells treated with EGF, in the absence (lane 1) and presence (lane 2) of synthesized peptide, using ab39688. 5-30µg of cell extracts loaded per lane.
Immunohistochemistry (Paraffin-embedded sections) - c-Myc antibody (ab39688)
ab39688, at 1/50 dilution, staining Human c-Myc in breast carcinoma tissue in the absence (left) and presence (right) of blocking peptide by Immunohistochemistry, Paraffin embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - c-Myc antibody (ab39688)
ab39688 staining P22 mouse dorsal skin tissue sections by IHC-P. Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 10% serum for 1 hour at RT. The primary antibody was diluted 1/20 and incubated with the sample for 24 hrs at 4°C. A biotinylated goat anti-rabbit antibody was used as the secondary in conjunction with streptavidin-Cy3®.
This image is courtesy of an anonymous Abreview
References for Anti-c-Myc antibody (ab39688)
This product has been referenced in:
- Xie XLet al. 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) promotes mouse hepatocarcinogenesis by activating transforming growth factor-ß and Wnt/ß-catenin signaling pathways. Toxicol Sci : (2011).Read more (PubMed: 22094457) »
- Lee SHet al. ERK activation drives intestinal tumorigenesis in Apc(min/+) mice. Nat Med 16:665-70 (2010). IHC-P; Mouse.Read more (PubMed: 20473309) »
See all 6 publications for this product
Publishing research using ab39688? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"