Anti-c-Myc (phospho S373) antibody (ab30643)

Overview

  • Product nameAnti-c-Myc (phospho S373) antibodySee all c-Myc primary antibodies ...
  • Description
    Rabbit polyclonal to c-Myc (phospho S373)
  • Conjugation notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Specificityab30643 detects endogenous levels of Myc only when phosphorylated at Serine 373.
  • Tested applicationsIHC-P, ELISA, IP more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human Myc around the phosphorylation site of Serine 373.

  • Positive control
    • IHC: breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab30643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use at an assay dependent dilution.
ELISA 1/20000.
IP Use at an assay dependent dilution.

Target

  • FunctionParticipates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
  • Involvement in diseaseNote=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
    Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
    Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence.
    Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
  • Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus.
  • Target information above from: UniProt accession P01106 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • bHLHe39 antibody
    • c Myc antibody
    • Cellular myelocytomatosis oncogene antibody
    • Cellular myelocytomatosis oncogene antibody
    • Class E basic helix-loop-helix protein 39 antibody
    • Myc antibody
    • Myc protein antibody
    • Myc protein antibody
    • Myc protein antibody
    • Myc proto oncogene protein antibody
    • Myc proto oncogene protein antibody
    • Myc proto-oncogene protein antibody
    • MYC_HUMAN antibody
    • Myc2 antibody
    • Myc2 antibody
    • Niard antibody
    • Niard antibody
    • Nird antibody
    • Nird antibody
    • Proto-oncogene c-Myc antibody
    • Transcription factor p64 antibody
    • v myc avian myelocytomatosis viral oncogene homolog antibody
    • v myc avian myelocytomatosis viral oncogene homolog antibody
    • v myc myelocytomatosis viral oncogene homolog (avian) antibody
    • v myc myelocytomatosis viral oncogene homolog (avian) antibody
    see all

Anti-c-Myc (phospho S373) antibody images

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma, using Phospho- Myc(Ser373) (ab30643). Suggested dilution: 1:50 - 1:100.

References for Anti-c-Myc (phospho S373) antibody (ab30643)

ab30643 has not yet been referenced specifically in any publications.

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