Anti-c-Myc (phospho S373) antibody (ab30643)

Overview

• Product nameAnti-c-Myc (phospho S373) antibodySee all c-Myc primary antibodies ...
• Description
  Rabbit polyclonal to c-Myc (phospho S373)
• Conjugation notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Specificityab30643 detects endogenous levels of Myc only when phosphorylated at Serine 373.
• Tested applicationsIHC-P, ELISA, IP more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthesized phosphopeptide derived from human Myc around the phosphorylation site of Serine 373.

• Positive controlIHC: breast carcinoma.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg⁺⁺ and Ca⁺⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • TGF beta
  • Nuclear

  • Cancer
  • Cell cycle
  • Cell differentiation

  • Cancer
  • Tumor biomarkers
  • Oncoproteins

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Transcription factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • HLH / Leucine Zipper

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

Applications

Target

• FunctionParticipates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
• Involvement in diseaseNote=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
  Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
  Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci.
• Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
• Post-translational
  modificationsPhosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence.
  Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex.
• Cellular localizationNucleus > nucleoplasm. Nucleus > nucleolus.

Target information above from: UniProt accession P01106 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4609 Human
  • Entrez Gene: 17869 Mouse
  • Entrez Gene: 24577 Rat
  • Omim: 190080 Human
  • SwissProt: P01106 Human
  • SwissProt: P01108 Mouse
  • SwissProt: P09416 Rat
  • Unigene: 202453 Human

  • Unigene: 2444 Mouse
  • Unigene: 12072 Rat

  see all

• Alternative names
  bHLHe39 antibodyc Myc antibodyCellular myelocytomatosis oncogene antibody

  Cellular myelocytomatosis oncogene antibodyClass E basic helix-loop-helix protein 39 antibodyMyc antibodyMyc protein antibodyMyc protein antibodyMyc protein antibodyMyc proto oncogene protein antibodyMyc proto oncogene protein antibodyMyc proto-oncogene protein antibodyMYC_HUMAN antibodyMyc2 antibodyMyc2 antibodyNiard antibodyNiard antibodyNird antibodyNird antibodyProto-oncogene c-Myc antibodyTranscription factor p64 antibodyv myc avian myelocytomatosis viral oncogene homolog antibodyv myc avian myelocytomatosis viral oncogene homolog antibodyv myc myelocytomatosis viral oncogene homolog (avian) antibodyv myc myelocytomatosis viral oncogene homolog (avian) antibody

  see all

Anti-c-Myc (phospho S373) antibody images

• 

  Immunohistochemistry (Paraffin-embedded sections) - c-Myc (phospho S373) antibody (ab30643)
  Immunohistochemical analysis of paraffin-embedded breast carcinoma, using Phospho- Myc(Ser373) (ab30643). Suggested dilution: 1:50 - 1:100.